Functions And Characteristics Of Duck Blood Hydrolysates And Its Primary Application

Duck blood is changed into nutritional compound including amino acids,small peptides,poly-peptides and heme by protease hydrolysis.The hydrolysate not only enhance digestibility,but also resolve the problem of hard to eat,and enrich the blood and iron.Using microencapsulation technology,could resolve the problems such as the material is difficult to preserve,bloody smell is strong,and so on.Besides,protecting active peptides of hydrolysate and preventing oxidation or transformation. In this paper, the basis nutrition component of duck blood hydrolysate and the basic function properties are studied by protease hydrolysis and microencapsulation technology,at the same time,the primary application is also studied,which supplying important academic bases for the exploitation of poultry blood.Detecting results show that dry duck blood hydrolysate is rich in protein,iron and zinc.The content of protein, iron and zinc is respectively 40.2%,484 mg/kg and 22.8 mg/kg.The results which research the molecular weight distribution of duck blood hydrolysate by the method of HPLC indicate that duck blood hydrolysate has lower 10000D average molecular weight,including 11 peptides of different molecular weight.The molecular weight of 8 peptides that in the scope of standard sample are10702,7788,6059,4113,3421,2417,1495,1065 D,small peptides content is 24.72%,and the molecular weight is less than 451 D.The results show that, the best formulation for duck blood hydrolysate microcapsules by piercing concreting method is:appending sodium alginate to duck blood hydrolysate without dilution or concentration,and its concentration 2% (w/v),the concentration of CaCl2 is 2% (w/v),the ratio of dropping phase and coagulating phase is 1:2,the encapsulation efficiency and loading capability reaches 91.6% and 40.2%.The dry duck blood hydrolysate microcapsule products have no bloody smell or peculiar smell,no viscid and fluid is good,the content of water is 5.12% and the density is 0.7 g/cm3,the diameter is between 371.1～488.0 m.The result of SEM indicates that microcapsules are global or elliptical particle,and the exterior structure is close-knit.The research results of sustained release properties of duck blood hydrolysate microcapsules in artificial gastric and intestine juice indicates,in artificial gastric juice(pH1.2),the release rate of duck blood hydrolysate microcapsules is fast in first 30min,along with time process,the release rate incline to slowness,cumulate release rate don't reach 30%;in artificial intestine juice(pH6.8),the duck blood hydrolysate microcapsules dissolve quickly after 1～2h,which explains that the duck blood hydrolysate microcapsules have pH-sensitive swelling property,and its sustained release effect is obvious.Short-term stockpile experimentation show that temperature has some influence in stockpile stability,but not obvious,the best condition is 4℃.Under the airproof condition of 4℃,the reserve rate of heme iron and water content are all keeping on a steady level after 6 month,explaining that the duck blood hydrolysate microcapsules don't deteroprate and sop up,and thus it can be seen that the microencapsulation technology have a good protection action for embeding duck blood hydrolysate,and the duck blood hydrolysate microcapsules have long-term stockpile stability.For discussing the primary application of the duck blood hydrolysate microcapsules,appending some microcapsules of duck blood power into crisp biscuit to fortify heme-iron is studied in this paper.The orthogonal test result is used to optimize the preparation conditions for crisp biscuit of duck blood power based on a single factor test as follows:duck blood power 3.3%,oil-fat 18%,sugar 36%,water 14%;soda 0.8%,ammonium bicarnate 0.2%;the best bake condition is:face-fire 190℃,underside fire 170℃,time 5min.The iron contest of this products is 3.67 mg/100g,and the correlative index such as water contest,basicity,acid value,peroxide value,are according to the request of GB/T 20980-2007, the products is favorable food for supplying iron nutriment.