Study On The Extraction Of Rice Peptide From Rice Feed Protein

This paper used rice feed protein as raw material, made a series of study about raw materials for the pretreatment, Enzymatic extraction of the protein and rice peptide, Refiner of rice peptide and analysis of product components. Designed to take full advantage of the rice feed protein resources, preparation of high-grade rice protein peptide, producing more and better products, improving science and technology level of traditional food and providing a strong basis and technical support for the depth exploiture and the use of rice resources.Key findings are as follows:Study direct and indirect effect of advanced micro comminution and washed pretreatment method to raw material particle size, content and the loss of protein. In the frequency of 80 Hz ,the smash time is 30 min, particle size can smash to about 800 head, D50=11.03μm. advanced micro comminution change in the size distribution of raw materials, promote the dissolution of active ingredient. Advanced aicro comminution with the crush of raw materials for enzyme, its extraction rate is 68.3%, 20 percent is better than extraction of raw materials. Raw materials by the washing, can remove the ineffective components.Increase the utilization of raw materials active ingredients. The best condition of washing is: liquid-solid is 7:1, the temperature of 60℃, time 60min, washed secondary. Under these conditions the extraction is up to 85.86 percent, and the loss rate of protein only 3.67%. Research has been using to the three-microbial protein enzyme which is commercialize- tion in China's current stage. Protease is used by the microbial activity, source of widespread, cheap and suitable for industrial production, and other features, will have broad application prospects. Determine the best extraction process: Basic protease -1.0%-0.4% neutral protease, acid protease -0.4%, solid-liquid is 1:10, starting pH10.5,50℃temperature hydrolysis 6 h,come to rice feed protein extraction rate of 74.58 percent.To remove the rice-liquid solution of salt and pigment for the purpose ,study the process conditions by system analysis of the use of activated carbon decolorization and yin and yang with ion exchange column desalination. The results showed that activated carbon and ion exchange column effective decoloring and desalination, to achieve the level of industrial needs. Optimum process conditions:①pH 6, the temperature of 50℃, the amount of activated carbon 2 percent, decolorizing 30 min.Decolo- rate reached 80.16 percent, the peptide loss rate to 8.27 percent.②enzyme solution to be 10 times the speed-volume flows through the ion exchange column of yin and yang, desalination rate of 82.18 percent and 80.92 percent and 8.27 percent rice peptide is loss.Use of amino acid analyzer instrument and gel products for amino acid analysis and the molecular weight arrangement of rice peptide. The total protein content of rice peptides products is 83.6 percent (dry weight), Which accounted for 75.7 percent peptide (dry weight), free amino acids accounted for 4.3 percent (dry weight).In the composition of the structure of peptide products, the majority peptide is composed by 3-6 amino acids, the molecular weight of the following in 1000. Rice peptide in the rate of 50.12 percent, 71.01 percent for the peptide purity. The solubility of rice peptides reached 98.53 percent. Peptide from the rice has a good solubility and color.