| Coal is a non-renewable hydrocarbon resource, and it is special chemical structure and characteristics. The direct application of coal not only brought environmental pollution seriously, but also led to wasting resources extremely. Bioconversion of coal has some advantages, such as milder condition and no pollution. It was new field of sciences. One of research focus was increasing bioconversional yield and choosing products. In order to improving bioconversional yield and choosing products, the coal was conversed by photo oxidation coupling with bioconversion.Taking Shenfu coal as participarts, different photo-oxidazed SFC was bioconversed by Monilia crassa Sh.Et Dodge, Penicillium citreo-viride Biourge and Flavobacterium Bergey et al,1923.97 which were extracted from Shenfu coal-washing waste water. Photo-biological co-conversion and the structure of bioconversion products were studied using FTIR and GC/MS. Further based on the experimental results that bioconversion of coal cased humic ,NAP and BPA. The mechanism of photo-biological co-conversional coal was proposed.Coal was pretreated by UV-photocatalytic oxidation in fixed bed photo-oxidation reactor. The results showed that the humic acid content of SFC photo-oxidazed for 6h was up to 10.91%,and 4h was up to 2.35%. The humic acid content of V-SFC photo-oxidazed for 4h was up to 5.30%, and 8h was up to 1.33%.The SFC photo-oxidazed for 6h and 4h .The V-SFC photo-oxidazed for 4h and 8h .The photo-oxidazed coal was extracted by sodium pyrophosphate alkali liquid. The residues were bioconversed in inorganic salts liquid medium. The results showed that SFC was conversed by photo-oxidazed for 6h coupling of Monilia crassa Sh.Et Dodge for 8 days, the total conversion yield was 39.72%, the yield of humic acid was up to 35.54% ,and V-SFC was conversed by photo-oxidazed for 8h coupling of Flavobacterium Bergey et al,1923.97 for 8 days, the total conversion yield was 30.80%,the yield of humic acid was up to 23.71%. It has coordination effect that photo-oxidation coupling with bioconversion.After photo-biological co-conversed, the products structure was characterized by FTIR. It was found that the content of aromatic was decreased, meanwhile the content of fat chains and oxygen-containing functional groups was increased in SFC.About V-SFC, the number of oxygen-containing functional groups was increased. SFC and V-SFC was photo-biological co-conversed, the products of water-soluble, precipitate treated by alkali and alkali soluble mainly contained–OH, amide, -N-O-,CH-(CH3) and ether.The residues that coal was conversed by photo-biological co-conversion. And the residues were extracted by propanone and THF. The structure of residues was characterized by GC/MS. After photo-biological co-conversion of SFC, the content of Benzene, 1-methyl-4-[(4-propylphenyl)ethynyl]- was maximum, and Phenanthrene, 1-methyl-7- (1-methylethyl) was second for the soluble extracted by propanone. After propanone extraction, the residuals were further extracted by THF. The content of Bis(2-ethylhexyl) phthalate was maximum, and dibutyl phthalate was second for the soluble extracted by THF. The solvent soluble matter of SFC which without photo-biological co-conversion didn't contain above four compounds.Model compounds and coal based humic acid were conversed by strains. The composition, structure and mechanism was studied.Base on above experimental results, the photo-biological co-conversional mechanism was presented.
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