| Magnetic polymer microsphere has been applied comprehensively in the biomolecule immobilization, separation, and magnetic targeted drug delivery. Recently, Various stimuli-responsive beads responding to temperature, pH, electric field, chemicals, and UV light have been attracted scientist's attention and employed to separate biomolecules or as a novel drug delivery system. Herein, Two kind of polymer beads composed of poly(methacrylic acid) (PMAA) and poly(acrylamide) (PAM) were first synthesized by the reverse emulsion polymerization respectively. Then Fe3O4 magnetic nano-particles were incorporated into the PMAA polymer beads to form the magnetic Fe3O4/PMAA composite particles. These polymer microspheres were characterized with Scanning electron microscope (SEM), Laser particle analyzer, thermogravimetric analyzer (TG) and Vibrating sample magnetometer (VSM). It was found that the magnetic Fe3O4/PMAA were spherical particles with 50μm average diameters. The microsphere was also pH-responsive with the volume change and monodispersed in aqueous. The proteins including IgG, BSA, SPA and Con A were successfully coupled to the magnetic Fe3O4/PMAA particle surface in different pH value. The results showed that maximum proteins can be coupled to microsphere with the highest coupling percentage at pH 7.0.0.2 mg of protein was coupled on 5 mg the magnetic polymer microsphere surface. On the other hand, this magnetic microsphere was also used in the plasmid DNA purification. In the drug carrier and release, the Doxorubici (DOX) and Levofloxacin (LVFX) were immobilized on the magnetic polymer microsphere surface and can be released at different pH value. The results indicated that the microsphere has potential applications in bioseparation and drug carrier. |
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