| Magnetic polymer nanoparticles has been applied comprehensively in the biomedicine research and magnetic targeted drug delivery. Recently, various stimuli-responsive beads (senstive to temperature, pH, electric field.etc) have been attracted scientist’s attention and used in bioseparation as well as a novel drug delivery system. Herein, the Fe3O4-PMAA magnetic nanoparticle has been synthesized by microemulsion polymerization. Thermogravimetric analysis, dynamic light scattering (DLS), transmission electron microscopy (TEM) and infrared spectroscopy (IR) show that the Fe3O4core surface evenly wrapped a layer of polymethacrylic acid. Fe3O4magnetic nanoparticles were incorporated into the PMAA polymer beads to form the magnetic Fe3O4-PMAA composite particles. It was found that the magnetic Fe3O4-PMAA is30nm in average diameters with spherical shape. The Zeta potential of the magnetic nanoparticles is-34.9.The magnetic nanoparticles were also pH-responsive with the pH value change in aqueous.When the pH at3.0, the Zeta potential of the magnetic nanoparticles is more than10. The cell lines HepG2were used as models to evaluate the cytotoxicity of the magnetic Fe3O4-PMAA nanoparticles.The cell viability was still89%when the nanoparticles concentrations reached in2000μg/ml. The results showed that the Fe3O4-PMAA magnetic nanoparticles have not evident cytotoxicity.In the drug loading and release study, the Doxorubici and Levofloxacin were selected to immobilize on the magnetic polymer nanoparticles surface and can be released at different pH value. This magnetic nanoparticles adsorption of the Doxorubici and Levofloxacin reached909.0μg/ml and362.1μg/ml. The results indicated that the nanoparticles has potential applications in drug delivery system. |
PDF Full Text Request