Preparation Of Nano-Complex By Ramification Of Benzylamine And Its Reciprocity With Proteins

Carbon nanotube is an important material as carrier which has particular applied value in the field of Biological and medical science.while, their characteristic nature of insoluble in any solvent, largely limits their application.Among so many modified methods of carbon nanotubes, we choose small amphiprotic molecule to modify carbon nanotubes by non covalent bond, so the disperstiveness of the modified carbon nanotubes in water will be increased.In this paper, we have quested the Preparation Conditions of the small amphiprotic molecule which can be a well surface active agent to carbon nanotubes. The glucolactone we used in the laboratory was available in the market, and its purity quotient is>99%,and another reactant is superfluous benzylamine (analytically pure). The reaction happened in absolute methanol under determinate condition.The carbon nanotubes we used in this paper is prepared by the method of CVD. It was purified by the aqueous solution of nitric acid which is a certain consistency. In this process, the excrescent impurityies at surface of carbon nanotubes were removed by the way of oxygenize, and the pure carbon nanotubes were received.In this paper, we used different consistency of methanol, ethanol, and isopropanol aqueous solution as the initial processed solvents of carbon nanotubes. And the processed carbon nanotubes dispersed again in the water to obtain a well dispersion, which were detectd by the ultraviolet spectrograph. So we can choose out the best initial processed solvents of carbon nanotubes. They are 15% methano,15% ethanol,30% isopropanol, in which the 30%isopropanol is the best one.The carbon nanotubes processed by the best initial processed solvents can adsorb BSA(Bovine Serum Albumin) in water. And the solutions were detected by ultraviolet spectrograph and the Circular Dichroism Spectrometer at three different temperatures(25℃,45℃,65℃). In addition to this, the changes of secondary structure in BSA (Bovine Serum Albumin) can be calculated by a soft named CDpro. So we can analyze the structure of BSA in ration.