The Establishment Of Quality Stands And Preparation Of Patchouli

Pogostemon cablin benth is a plant belonging to the Labiatae. The main components of Pogostemon cablin benth is patchouli oil. Patchouli oil is more than 30 kinds of proprietary Chinese medicines, such as "Huo Xiang Zheng Qi Wan (Water)", "anti-virus oral" and "HUODAN pill" and other important component of drug. In addition, patchouli oil also can be used as cosmetics, deodorants, and insecticides, setting the production of ingredients. It is an important export product. In order to give full play to the famous South patchouli oil, It is need for the quality of patchouli oil, Quality Standards and Fingerprint of essential oil have been investigated t. The preparation of pogostone has been studied.1 Study on Characters of Patchouli oilPatchouli oil is reddish-brown or green brown clear liquid. It has a specific aroma.It is pungent and tepid. It can be solubled with chloroform, ethyl ether and petroleum ether in any proportion. According to Chinese Pharmacopoeia 2005 edition, we amendment the Determination of refractive index base at the original standard. Relative density, optical rotation, refractive index were determined. The results meet the requirements 2 Study on TLC of Patchouli oilPatchouli alcohol and pogostone were used as Reference substance. High performance thin layer silica gel GF254 plates. Petroleum ether:Ethyl acetate:Eormic acid (10:0.7:0.6) were as Expand agents.18℃, Relative humidity was 30%.5% Ferric chloride was used as Reagent. We established TLC method of patchouli oil.Meet the quality standards requirements.3 Simultaneous determination of contents of patchouli alcohol and pogostoneThe GC approach was developed for quantitatively analysis of two compounds in patchouli oil.10 samples were collected from 6 factories in China and the other two samples were preparation by ourselves, they were analyed and the results indicated that total contents of the two compounds were significant different in different batches. The results showed multiple chemical markers provided more comprhensive information than unique marker for quantitative analyses of patchouli oil.4 Study on GC fingerprinting of patchouli oilThe GC fingerprinting of patchouli oil has been established by GC. The similarity of all samples were evaluated by similarity evaluation software(2004A) published by committee of condex According to the analysis of fingerprint of the 12 batches of samples from different areas, The fingerprints of every two batches have high similarity. The differences in the relative contents of characteristic peaks lead to diverse outline of GC chromatography for different samples.5 Study on the preparation of pogostoneWe investigate the antibacterial activity of patchouli oil,patchouli alcohol, pogostone and no pogostone part of the patchouli oil. The results showed that the pogostone is the main Antibacterial. It was reported that pogostone has insecticidal activity. There are no related reports of Mass production about the compound. There are no product in market.we study on the mass preparation of pogostone. Patchouli oil was used as the raw materials.The results show that the process is short. The equipment is simple. the cost is low. It is easy to operate. The purity of the product is high.