Use Of Thiolated-β-cyclodextrin Modified Gold Nanoparticles For Chiral Separation Based On Capillary Electrochromatography

Capillary electrophoresis is a useful separation technology and has been applied to many Fields,owing to its characteristics:high efficiency separation,high speed analysis,more manipulation mode and et al.Applications of thiolated-β-cyclodextrin modified gold nanopar- ticles for chiral separation based on capillary electrochromatography were studied in this article which aimed at establishing an analysis method of efficiency separation,high speed analysis and low consumption.It includes some parts in this research work:(1)A classics method was introduced in synthesizing thiolated-β-cyclodextrin modified gold nanoparticles of which were water soluble,homogenized and good stability.And they were characterized by TEM,NMR,FT-TR and UV absorption spectroscopy.(2)Use the synthesized of thiolatedβ-cyclodextrin modified gold nanoparticles as pseudostationary phase for amino acid enantioseparation based on capillary electrochromato- graphy was studied in this chapter.Experimental conditions of buffer pH,separation voltage and concentration of GNPs were optimized.Baseline separation was received at very low concentration of GNPs(such as 1.0mg/mL,to be equivalent to 0.38 mM ofβ-CD).(3)Use the synthesized of thiolatedβ-cyclodextrin modified gold nanoparticles as pseudostationary phase for drugs enantioseparation based on capillary electrochromatography was studied in this chapter.Experimental conditions were optimized.Baseline separation was received at very low concentration of GNPs(such as 0.8mg/mL,to be equivalent to 0.30 mM ofβ-CD).(4)Some inorganics and organics in explosives residue were analyzed by capillary electrophoresis.It could be confirmed accurately that the kind of explosives by experiment which could apply clues and evidences for cases of exploding.