The Effect Of Fe On The Growth Of PSB And The Synthesis Of Photosynthetic Pigments

Nowadays the application of photosynthetic bacteria is relatively wide and is involved with such many fields as agricultural production and aquiculture. One important application of them is to treat environmental pollution, including treatment of water pollution, water purification, control over forming and development of red tide as well as rehabilitation of water body after red tide. But these problems get involved in fairly high iron content. Whether or not photosynthetic bacteria adapts to such high iron concentration environment and how does photosynthetic bacteria grows, which is a basic point to apply photosynthetic bacteria into this field.In addition, the iron in water contains Fe3+, Fe2+, inorganic iron ion, organic and complex iron, so which one of them is more contributive to the growth of photosynthetic bacteria and is the key utilization form? As for aforementioned problems, this thesis carries out the following design and research.This issue involves two kinds of Fe(Ⅱ) and four kinds of Fe(Ⅲ), of which Fe(Ⅱ) includes one kind of inorganic molysite(FeSO4·6H2O)and one kind of dissolved organic ligands molysite [FeSO4·7H2O,FeSO4·(NH4)2SO4·6H2O]; Fe(Ⅲ) includes two kinds of inorganic molysite [FeCl3·6H2O,Fe2(SO4)3·xH2O] and two kinds of dissolved organic ligands molysite [C6H5FeO7·H2O,EDTA-Fe·3H2O]. Each kind of malysite contains culture medium respectively with 0,100,200,200,300,400 to 500μmol/L six concentration gradient. Try to analyze the effects of Fe on the growth of photosynthetic bacteria and the concentrations and types of photosynthetic pigment, through observation of growth of Rhodobacter az134k20 bacteria, measurement of biomass and 660nm absorption spectrum, extraction & thin-layer chromatography of photosynthetic pigment and measurement of absorption spectrum under different types of malysite and concentrations.The result is as follow. Seen from the growth of bacteria, Rhodobacter az134k20 bacteria fast grows in Fe(Ⅲ) culture medium than in Fe(Ⅱ) culture medium and the lag phase is shorter; the growth of bacteria under dissolved organic ligands Fe molysite culture medium is faster than that of inorganic Fe molysite culture medium and the adjustment period is shorter. Seen from bacteria biomass, the biomass of Rhodobacter az134k20 bacteria in Fe(Ⅲ) culture medium is more than that in Fe(Ⅱ) culture medium, while the biomass of bacteria in dissolved organic ligands molysite is more than that of inorganic molysite culture medium.Analysis of photosythetic pigment of Various cultures shows that the concentration and the kind of malysite does not have obvious effect on content and species of Crt. When the concentration of malysite ranges from 0-400μmol/L, the content of Bchl increases as the increment of the concentration of Fe. Bchl of the bacteria under the 400μmol/L and 500μmol/L Fe have the approached content. However, the two main components of Bchl do not change along with different kinds and concentration of malysite.