Study On The Curcumin's Complex Reaction With Iron By UV-VIS And The Qulity Control Of Chlorhexidine Hydrochloride In Production Process By HPLC

There are four parts in this thesis. Details are as follows:In the first chapter, the applications of Ultraviolet-Visible Spectrophotometry and High Performance Liquid Chromatography in pharmaceutical analysis are reviewed, and the research background is introduced.In the second chapter, curcumin's complex reaction with Iron was studied, and a method was established for the determination of Iron (Ⅲ) in tap water by UV-VIS.Curcumin is a natural phenolic substance contained in the rhizome of Curcuma longa. It has been reported to possess a variety of pharmacological activities such as antioxidant, anti-cancer, anti-inflammatory, no toxicity. It is an ideal "green" ions color reagent. At the higher concentration, polymerized curcumin is more sensitive to complex with Fe (Ⅲ). The molar absorptivity is 3.86×104 L·mol-1·cm-1, which is higher 3 times than Phenanthroline spectrophotometry. The proposed UV-VIS method was established for the determination of Iron (Ⅲ) in tap water. The average recovery of Iron (Ⅲ) was 101.7%. It is simple, sensitive, selective, and suitable for the determination of Iron (Ⅲ) in tap water.In the third chapter, a method was established for the determination of Iron(Ⅱ) in Compound Ferrous Sulfate and Folic Acid Tablets by curcumin spectrophotometry.Compound Ferrous Sulfate and Folic Acid Tablets are the compound preparation composed of Ferrous Sulfate, folic acid, dry yeast, Angelica, Astragalus, Largehead Atractylode, et al.. It is a antianemic agent. Based on the previous chapter, a method was established for the determination of Iron (Ⅱ) in the tablets by curcumin spectrophotometry. The average recovery and RSD of Iron (Ⅱ) were 101.6% and 1.7%, respectively. The molar absorptivity is 3.69×104 L·mol-1·cm-1, which is higher 3 times than Phenanthroline spectrophotometry. The method is convenient, accurate, sensitive, and suitable for the quality control of Compound Ferrous Sulfate and Folic Acid Tablets.In the forth chapter, the quality control of Chlorhexidine Hydrochloride in the production process was studied by HPLC.The analysis was carried out with ODS column (150 mm×4.6 mm,5μm), using methanol:0.06 mol·L-1 KH2PO4-0.07 mol·L-1 H3PO4 (54:46, V/V) as the mobile phase. The flow rate was 0.8 mL·min-1, the detection was set at 240 nm and the column temperature was maintained at 35℃. Under this conditions, each compound's peak shape was symmetrical and the separation was good. The correlation coefficient of Chlorhexidine Hydrochloride and p-Chloroaniline were 0.9999, the average recoveries were 101.1% and 100.5%, respectively. The proposed method has good selectivity and sensitivity, and can be used for the quality control of Chlorhexidine Hydrochloride in the production process and daily.