Study On The Process To Seperate Antioxidant Polysaccharides From Auricularia Auricula By Radial Chromatography

Auricularia auricula is the precious medicinal and edible gelatinous fungi, and extremely rich in nutritional and medicinal value. Polysaccharides in Auricularia auricula are potential biological ingredients. According to a large number of studies, polysaccharides in Auricularia auricula possess bioactivities in regulating immunity, reducing blood lipids, anti-aging, anti-oxidation, anti-tumor, anti-coagulation and anti-thrombotic. Radial chromatography is one of the most effective instruments of rapid separation and preparation of complex samples. Because it adopts the radial flow technology, samples and mobile phase flow from the surrounding of the column to the center of a circle or from the center of a circle to the surrounding, with rapid speed, low pressure, easy to operate, easy to linear amplification, etc., which solves the problems of the traditional axial chromatographic technique, and has a great advantage of the separation and purification of biological macromolecules. In order to more effectively purify polysaccharides in Auricularia auricula and get higher antioxidant activity and higher purity polysaccharides in Auricularia auricula, this paper studied on the application of radial chromatography on the separation and purification of polysaccharides in Auricularia auricula.Decolorization of polysaccharides in Auricularia auricula was studied. The results showed that the resin adsorption was an excellent decolorating method. After decolorization, polysaccharides retention rate was 68.6%, protein removal rate was 55.8%.The effects of adsorption and desorption of polysaccharides in Auricularia auricula on DEAE anion-exchange resin were studied based on experiments of static adsorption, dynamic adsorption and desorption. The results showed that the concentration and pH value of the sample solution were the key factors of the resin exchange capacity, and the concentration of the eluent influenced the elution curve. The optimum sample concentration was 2.5 mg/mL, the optimum pH was 7.0. Under the optimum condition adsorption capacity of the polysaccharides was 5.69 mg/mL. The concentration of eluent from 0.1 mol/L to 0.5 mol/L gave a good separation result.AAPII, a polysaccharide component with a higher antioxidant activity from a separation by Toyopearl DEAE-650 M, was selected as the target product of polysaccharides in Auricularia auricula of radial column chromatography with Toyopearl DEAE-650 M.Through comprehensive analysis of single factor experiments, the optimum operating conditions were obtained as fllow:sample volume, 200 mL; sample amount,2000 mg; pH value,7.0; flow rate of sample load and elution,45 mL/min; and temperature,20℃.The seperation of polysaccharides in Auricularia auricula were compared bewteen radial and axial chromatographic technique. The results indicated that radial chromatography column had lower pressure with large flow operation, and the seperation efficiency improved markedly, much faster than axial chromatography column. Radial chromatographic technique was much easier to linear amplification, which can be used for the separation and purification of large-scale production.The above research results will be able to provide technical support for the large-scale purification of polysaccharide AAPⅡfrom Auricularia auricula with radial chromatography, and also provide scientific basis for other polysaccharides.