Analysis And Research Of Extraction Method Of VB₂ In The Grains

Vitamin B₂ is the composition of flavin mononucleotide and flavin adenine dinucleotide which are the coenzyme of flavin enzymes, In the biological oxidation process, it can play the role of transferring Hydrogen, and participating in metabolism of sugar, protein and fat in the body. It is long-term lack of vitamin B₂ that the glossitis, stomatitis, mouth rot, eye fatigue, cataracts and other diseases will appear. Vitamin B₂ can not be synthesized in the body, but need to be the exogenous food supplied. The grain is one of important sources of vitamin B₂, therefore, to establish a simple, accurate and rapid determination method of vitamin B₂ in grain has important practical significance.Currently, many methods with using fluorescence to determine vitamin B₂ in vegetables, tablet were reported, however, the effect of the method with determining vitamin B₂ in vegetables, tablet was used to extract vitamin B₂ in grains was not satisfactory.The extraction of vitamin B₂ has been improved and optimized in the text, and effect of the different extraction, extract method and the condition of extraction on VB₂ in the millet was studied through single factor trial and orthogonal design trial. By adopting fluorescence luminosity law, the research measured content of vitamin B₂ in millet onλex=444.0nm andλem=528.0nm. The result indicated that the quantity of VB₂ extracted was higher when the experiment selected H3PO4 for extraction and used the water bath backflow. The effect of extraction was also better when extraction density was 0.1 mol/L, extraction time was 45 min and pH of solution was 5.0. And the text recovery reached from 89.20% to 100.32%, and the coefficient of variation was controlled by 1.21%. Hence, Precision and Nicety of this method was very fine. A new method for quick and exact determination of the VB₂ in millet was offered.Under the effect of salt, water-soluble small molecule organic solvent can form two-phase system for extraction of target substances. Based on this a new method was put forward with isopropanol-salt-water aqueous two-phase extraction for extraction vitamin B₂, Effect of different organisms, different organic amount, pH, and added a small amount of salts on the ability of separation phase of isopropanol alcohol was studied. The results showed that: under optimum conditions, one-time extraction rate of isopropanol-MgSO4 system to vitamin B₂ was 97.6% to 98.4%, and the detection limit was 1.6×10-2mg/L. the relative error of the method used for the determination of vitamin B₂ in rice was controlled by 1.22%. With the traditional liquid-liquid extraction and solid phase extraction compared, the method is simple, raw materials with cheap and abundant, interference with small, low toxicity, low power consumption and other significant features.