Applications Of Macrocyclic Complex Catalyzed Oscillating Chemical Reactions In Pharmaceutical Analysis

Chemical oscillation could reveal the phenomenon of nonlinearity and nonequilibrium which existed in the nature, and it has attracted considerable attention and extensive study of researchers in recent years. At present, the research for the oscillation chemistry mainly focus on the design of new oscillator, the upbuild of mechanism model and the application on the analytical determination. In this paper, we set up a new bromate oscillating system which involves macrocyclic complex [CuL](ClO4)2 as catalyst and malic acid as organic substrate, and studied the disturbing effect of medicine on this system.Firstly, The catalyst macrocyclic complex [CuL](ClO4)2 was synthesized according to literature and was identified by I.R. spectrum and elemental analyses. The ligand L in the complex is 5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazacyclotetra deca-4,11-dine. Then, the stable bromate oscillating system:NaBrO3-malic acid-[CuL](ClO4)2-H2S04 was established. When the Isoproterenol was injected into the system, it disturbs the oscillation and results in an increaser oscillation amplitude. The change of the oscillating amplitude is linearly proportional to the logarithm of the Isoproterenol. A new analytical method for the determination of Isoproterenol was proposed. The possible perturbation mechanism of Isoproterenol on the oscillating system is also discussed.The effect of the Levodopa on the [CuL](ClO4)2-catalyzed oscillating reaction was studied. When the Levodopa was injected into the system at the bottom of the potentionmetric cycle, the potential dropped gradually to a minimum and then increased sharply to normal oscillation. In the range of 9.90×10-6 to 2.44×10-4 M, the concentration of Levodop was linear to the change of amplitude with a correlation coefficient of 0.9933. A new analytical method for the determination of Levodopa was proposed.A novel methodological approach for the determination of PAS-Na based on its perturbation effect on [CuL](ClO4)2-catalyzed oscillating reaction was proposed. Under the optimal conditions, different of PAS-Na concentrations were injected into the system. The results show that the changes of the oscillating amplitude is linearly proportional to the PAS-Na concentration in the range 4.97×10-7 to 1.96×10-4 M, with the correlation coefficient being 0.9915. The detection limit is 1.96×10-7 M. And the effects of some foreign species were studied.