Studies On Extraction Procedure Of Soluble Dietary Fiber From Grape Waste

Plant dietary fiber is a functional food additives, and has special physiological functions of the sum of plant components on the body, including a variety of cellulose and lignin, plastic and other materials. Long-term consumption of foods rich in dietary fiber has a significant effect to prevent such as colon cancer, cardiovascular disease, hypertension and other diseases. Therefore, great prospects can be found on research and utilization of new sources of dietary fiber prepared from different sources, different kinds of dietary fiber.One-year-old canes and pomaces of Cabernet Sauvignon as the test material, this thesis explored the extracting procedure with HCl or NaOH or enzyme for soluble dietary fiber from test materials to get the best combination methods of soluble dietary fiber. The soluble dietary fiber contents from the canes of 42 grape varieties and 8 grape pomaces from 8 varieties were determinted. Specific contents include the following aspects.(1) With the aim to access the optimum procedure, single-factor tests were conducted to extract samples of soluble dietary fiber with different HCl solutions. The infected factors were the concentration of HCl, extracting temperature, extracting time and solid to liquid ratio. The results of L9(34) orthogonal test with HClto extract the dietary fiber from grapevine canes indicated that the concentration of HClwere marinated in 7%(v/v), 1︰15 of the ration of material to liquor, 85℃for 100min. The results of L9(34) orthogonal test with HCL to extract the dietary fiber from grapevine pomaces indicated that the concentration of HCl were marinated in 1.2% (v/v),1︰20 of the ration of material to liquor, 75℃for 75min.(2) With the single factors as the concentration of enzyme, extracting time, extracting temperature and solid to liquid ratio, a single-factor test was conducted to access the optimum procedure. The results of L9(34) orthogonal test with enzyme to extract the dietary fiber from grapevine one-year-old canes indicated that the concentration of enzyme were marinated in 9.0%(g/v), 1︰20 of the ration of material to liquor, 80℃for 105min. The results of L9(34) orthogonal test with enzyme for extracting the dietary fiber from grape pomaces indicated that the concentration of enzyme were marinated in 1.4% (g/v), 1︰10 of the ratio of material to liquor, 55℃for 140min.(3) The aim was to access the optimum procedure extracting samples of soluble dietary fiber with different NaOH solutions, which was the results of single-factor test. The infected factors were the concentration of NaOH, extracting temperature, extracting time and solid to liquid ratio. The results of L9(34) orthogonal test with NaOH to extract the dietary fiber from grapevine canes indicated that the concentration of NaOH were marinated in 7%(v/v), 1︰15 of the ratio of material to liquor, 85℃for 100min. The results of L9(34) orthogonal test with HCL to extract the dietary fiber from grapevine pomaces ndicated that the concentration of HCL were marinated in 1.2% (v/v),1︰20 of the ration of material to liquor, 75℃for 75min.(4) With three optimum procedure to extract the soluble dietary fiber from 42 different grapevine canes and 8 different grape pomaces, the results of variance analysis indicated the difference of soluble dietary fiber contents from different grapevine canes and pomaces was very significant.(5) Ten compositions for 42 kinds grapevine canes and 8 kinds of pomaces including soluble protein, carbohydrate, ash, total dietary fiber, soluble dietary fiber, insoluble dietery fiber, pectin, cellulose, hemicellulose and lignin were determinated. The results of analysis of variance indicated the difference of contents from different grapevine canes and pomaces was very significant.