Chemical Constituents From The Rhizomes Of Helleborus Thibetanus Franch.(Ⅲ)

Tiekuaizi, the rhizomes of Helleborus thibetanus Franch., is a kind of Chinese traditional herb medicines in china, and has a clear effect. H. thibetanus is a Chinese endemic specie which mainly distributes in Qinling Mountains in China. The research on its effective chemical constituents has rarely been reported. Based on the theories and principles of phytochemistry, the chemical constituents of H. thibetanus from Taibai Mountain was studied.This thesis introduced the distribution and biological characteristics of H. thibetanus, summarized the progress studying on bufadienolides, also chemical constituents and pharmacological researches of Helleborus species.The systematical test on chemical constituents in H. thibetanus showed that the plant contained phenolic composition, flavonoids, steroid saponins, terpenes, tannins, carbohydrates, organic acids and volatile oils etc., and did not coumarined, anthraquinones, alkaloids, cyanogenic glycosides.Nine compounds were isolated and purified from 70% ethanolic extract of H. thibetanus by silica gel, Si60, D101 macroporous adsorption resins, Sephedex LH-20 column chromatography, and recrystallization. Based on the analysis of their spectral data (UV, IR, MS, 1H NMR, 13C NMR, HMBC, HSQC, ROESY) and compared with the related literatural data, the nine compounds were identified. Three phytoecdystones:β-ecdysterone (TGC-1), polypodine B (TGC-2),α-ecdysone (TGC-8). One flavanoid: 5-hydroxy-7- methoxyflavanone (TGC-3). One small molecular compound: veratric acid (TGC-5). Four bufadienolides: deglucohellebrin {3,5,14-trihydroxy-19-oxo-3-[(α-L-rhamnopyranosyl)oxy] bufa-20,22- dienolide} (TGC-6), hellebrigenin (TGC-7), tigencaoside A {14β,16β- dihydroxy-3β-[(β-D-glucopyranosyl) oxy]bufa-20,22-dienolide} (TGC-9), tigencaoside B {14β,16β-dihydroxy-3β-[(β-D-glucopyranosyl-(1→4)-O-β-D-glucopyranosyl)oxy]bufa-20,22-dienolide} (TGC-10). Compounds TGC-9 and compound TGC-10 was newly discovered.