Optimizing And Researching Of Yeast Fermentation Of Sweet Stalk Of Ion Implantation Condition

The fuel ethyl alcohol is one kind of the clean energy , in its production the yeast mold mushroom spawn is the entire production system's core. The effect of different pouring dosage on survival rate of yeast when feeble-minded N+ was poured into the mutafacient saccharomycetes were studied, and the yeast strains of high production of ethyl alcohol were screened. The main research content was as follows:1. The sweet sorghum straw stalk juice domestication's yeast strain SC-71 was mutated with feeble-minded N+, the effect of different dosage in certain energy on survival rate and positive and negative mutation rate of SC-71 was studied. And when the dosage was about 220×2.6×10¹³ N⁺ion/cm² the saccharomycetes have the higher survival rate, its survival rate was about 6%. When the dosage was about 200×2.6×10¹³ N⁺ion/cm², saccharomycetes' rate of mutation was the highest, its mutation rate was about 25%.2. To passes through feeble-minded N+ to pour into induces mutation or chromosomal change and inherits stable yeast strain 90# to carry on the redundant mutagenesis which feeble-minded N+ pours into, studied the energy was certain when has poured into the dosage to embark differently the strain 90# survival percentage and the positive and negative rate of mutation influence. And in pours into the dosage for 180×2.6×10¹³ N⁺ion/cm² about time the saccharomycetes have the high survival tate, its survival rate about 3%. But pours into the dosage about 200×2.6×10¹³ N⁺ion/cm² time, saccharomycetes' rate of mutation is the highest, its mutation rate was about 12%.3.Pours into mutafacient after feeble-minded N+ strain 90# and SC-71 carry on progressive screening, finally obtains five use sweet sorghum straw stalk juice fermentation production fuel ethyl alcohol high production strain SCNN-1, SCNN-2, SCN-1, SCN-2, SCN-3. In the sweet sorghum straw stalk juice's sugar hammer is 20%, pH is 5.0, vaccination quantities is 1%, fermentation temperature for 28±1℃the condition, in the fermentation unclear wine fluid's alcohol content respectively is 10.26, 10.19, 10.13, 10.18, 9.94%, the residual sugar content respectively is 0.31, 0.34, 0.28, 0.33, 0.35g/100ml.