| The ordered mesoporous carbon (OMC) possesses large surface areas, narrow pore sizedistributions, ordered structures, showing great potential in biomedical applications andtherefore have attracted considerable attention. The obtained ordered mesoporous carbonsamples were characterized by XRD, TEM, SEM and N2 adsorption–desorption.Firstly, Ordered mesoporous carbon (OMC) functionalized with poly-azure B (denotedas PAB/OMC) composite-modified electrode was fabricated by electropolymerizing azure B(AB) on the surface of glassy carbon electrode (GCE). The new nanocomposite film wascharacterized by cyclic voltammetry (CV) and scanning electron microscopy (SEM). Theresults indicated that this poly nanocomposite modified electrode had strong electrocatalyticactivity toward the oxidation of dihydronicotinamide adenine dinucleotide (NADH). Underthe selected conditions, the anodic peak current of NADH was linear with its concentrationwithin the range from 3.00 to 1000μM, and the detection limit was 0.10μM (S/N=3). Thelow detection potential of the nanocomposite film (+0.045 V) also eliminated interferencesfrom other redox-active molecules such as uric acid (UA), dopamine (DA) and ascorbic acid(AA). In comparison with the OMC/GCE or PAB/GCE electrode prepared in the similar way,this composite-modified electrode exhibited better catalytic activity, selectivity and linearrange.Secondly, glucose oxidase (GOD) was adsorpted on the surface of OMC modified glassycarbon electrode (GOD/OMC/GCE). Then ferrocene was assembled on the GOD/OMC/GCEplatform to construct an amperometric glucose biosensor. The results showed that theFc/GOD/OMC nanocomposite exhibits a remarkably improved catalytic activity towards theoxidation of glucose. The biosensor exhibited a wide linear response up to 10.00 mM with alow detection limit of 1.03±0.15?μM (S/N=3). The selectivity and stability of the biosensorwere also investigated.
|
PDF Full Text Request