The Synthesis Of Rhodamine, And Coumarin Derivatives And Investigation Of Their Spectra Properties

In recent years, as a Commonly used dyes, the rhodamines and coumarins have attracted intense attention of its unique photophysical properties. As fluorescent groups, rhodamines more and more used in biological experiments, with its good water solubility, the excitation wavelength long, non-toxicity advantages, more and more used in biological experiments. In addition, coumarins are also excellent optical performance fluorescent dyes, they are often used as a separate light-emitting dyes can also be used as energy transfer donor.Previous studies in this laboratory, we have designed and synthesized a number of rhodamine derivatives used as probes. However, in our past work, we did not study all the rhodamines derivatives dye of a series light-emitting properties of light to make a specific comparison as systems. Thus, on this basis, we continue a synthetic derivatives of various rhodamine dyes, and to compare the spectral properties and research. Meanwhile, we use coumarin in various properties, design and synthesis of coumarin Jacquelina of Co2+ and Ni2+ sensor. At last, we take coumarin as a donor and rhodamine as the chromophore design and synthesis of the Fe3+ ion probe.This thesis aims to understand the types of coumarin and spectral properties of rhodamine derivatives and the nature of its spectrum to design and synthesize a better sensor. At first, we provided a rare example of a turn-on fluorescence chemosensor for paramagnetic Ni2+ and Co2+. Then we take Rhodamine-coumarin developed as the first ratiometric fluorescent sensor for Fe3+ based on regulation of FRET. This compound was readily synthesized in one step. The sensor displays a significant fluorescent emission enhancement at 575 nm with a slight fluorescent emission increase at 420 nm in the presence of Fe3+. The ratio of emission intensities at 575 and 465 nm (1575/1465) varied from 0.014 to 1.05, corresponding to a 75-fold enhancement upon Fe3+ treatment. These highly favorable ion probes can be used as an important tool to study in various organisms.