Sterilization Mechanism Of Acidification Of High Pressure Carbon Dioxide

High pressure CO2 sterilization technique is a new cold sterilization one that has been developing in recent years. It has several advantages such as low sterilization cost, no toxicity reaction, good sterilization effectiveness and strong maneuverability and so on. Far from 1990s, the research of high pressure CO2 sterilization technique has been taken more and more seriously by domestic and overseas scholars, but the research of high pressure CO2 sterilization mechanism has been remained guess because of no experimental data.In order to find out the acidulation mechanism in all major sterilization mechanism, this paper made use of indirect experimental methods to test acidulation Sterilization mechanism. According to the principle that F-ATPase (membrane-bound proton-translocation ATPase) and P-ATPase (plasma membrane ATPase) can adjust intracellular proton concentration automatically to ease the microorganism detrimental influence from exotic acidification, this paper can be designed. Taking the Escherichia coli as researchful bacteria, the experiment made use of the method of combination of ultraviolet radiation and inhibitor to build low activity P-ATPase and low activity H+-ATPase-defective mutant; made use of one-way orthogonal design to educe the optimal sterilization conditions of high pressure CO2; made use of the combination result of sterilization effectiveness of high pressure CO2 to normal Escherichia coli and mutant bacteria, the influence of H+-ATPase activity to high pressure CO2 sterilization, and the significance analysis of H+-ATPase to the significant effect of high pressure CO2 to go on preliminary study about sterilization mechanism of acidification of high pressure CO2.The study result showed that H+-ATPase activity had significant effect to the sterilization effectiveness of high pressure CO2, and the sterilization effectiveness of high pressure CO2 to low activity H+-ATPase mutant strain was better than the sterilization effectiveness of high pressure CO2 to normal Escherichia coli, which explained that acidification mechanism had played an important role in the sterilization process.