| Recently, there has been a rapid growth in the development of the highly selective and sensitive optical nanobiosensors based on gold nanoparticles modified films for disease diagnosis, drug discovery, proteomics and molecular biology.Taking polyvinylpyrrolidone (PVP) as protective agent and sodium citrate as reducing agent, homogeneous gold sols were prepared by chemical reduction in this paper. DL-1,4-dithiothreitol (DTT) was first self-assembled on a flat gold plate electrode with a surface area of 5mm×5mm, 3-mercaptopropionic acid was chosen to bind with gold partics to form molecular recognition biosensors in comparison with that of L-cysteine. The prepared gold nanoparticles were charactered with UV-Vis and TEM. And their assembling processes were tested by cylic voltammetry.Broken through the traditional catalysis of EDC-NHS, the EDC-DMAP as a nice catalyst of interaction between amino and carbonoxyl groups has been exploited. The nanobiosensor, based on the gold nanoparticles modified with the organic functional groups with activity increased, exhibited distinct optical properties of localized surface plasmon resonance (LSPR) in visible absorption region. The maximum adsorption amount of c-myc monoclonal antibody molecule on the modified LSPR nanobiosensor with the novel organic functional nano film could reach 2.4μg/mL.The LSPR biosensor immobilized with c-myc monoclonal antibody could be used for recognition of oncogene biomolecules such as MYC recombinant protein (P01) with a low linear response range of 6.5×10-3—1.3μg/mL and a detection limit of 6.5×10-3μg/mL. No significant interferences from bovine serum albumin (BSA), swine hemoglobulin, bovine albumin, transferrin, human hemoglobulin, thrombin, lysozyme could be observed. In comparison with enzyme linked immunosorbent assay (ELISA), the LSPR nanosensor possessed a recovery of 92.31%—109.23% for determination of samples containing c-myc protein with nice reproducibility, showing its promising feasibility. Moreover, the presented nanobiosensor has been successfully applied to detection of amount of c-myc protein in tumor cells, showing its potential application in anticancer predictive and therapeutic strategies.The LSPR biosensor immobilized with c-myc recombinant protein could be used for recognition of c-myc oncogene related biomolecules such as DNA plasmid with a low linear response range of 9.0×10-3—1.5μg/mL and a detection limit of 9.0×10-3μg/mL. No significant interferences from bovine serum albumin (BSA), swine hemoglobulin, bovine albumin, transferrin, human hemoglobulin, thrombin, lysozyme could be observed. In comparison with enzyme linked immunosorbent assay (ELISA), the LSPR nanosensor possessed a recovery of 91.11—112.00% for determination of samples containing DNA plasmid with nice reproducibility, showing its potential application on biomedical and molecular biology.The LSPR biosensor immobilized with c-myc monoclonal antibody could be used for recognition of c-myc oncogene related biomolecules such as anti-mouse IgG (H+L) antibody with a low linear response range of 4.0×10-3—0.20μg/mL and a detection limit of 4.0×10-3μg/mL. No significant interferences from bovine serum albumin (BSA), swine hemoglobulin, bovine albumin, transferrin, human hemoglobulin, thrombin, lysozyme could be observed. In comparison with enzyme linked immunosorbent assay (ELISA), the LSPR nanosensor possessed a recovery of 91.00—110.00% for determination of samples containing anti-mouse IgG (H+L) antibody with nice reproducibility, showing its potential application on biomedical and molecular biology.
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