| The drainage of high sulfate waste water resulted in serious ecological problems. Thereofre, all nations in the world have promulgated strict laws to forbid high sulfate waste water draining. At the same time, sulfate waste water bio-treatment technique was born.Because the anaerobic baffle reactor(ABR) has synthesized the advancements of single-phase anaerobic reactor and two-stage anaerobic reactor. It shows that the treatment of low, center and high concentrated organic waste water can achieve better effect. As the unique structure characteristic and the processing capacity of ABR, it has received many scholars'focusing attention on, in view of the fact that carried on an experiment to deal with the containing sulfate organic wastewater with ABR.In this work, the operation effectiveness of acidogenic sulfate-reducing reactor aeffected by the sulfate and alkalinity was studied and discussed. The micro-organism community dynamics influenced by the alkalinity and sulfate was analysed with the help of the PCR-DGGE. At the same time, the operation effectiveness of sulfate reducing reactor and the micro-ogranism community dynamics reacted by the carbon resources was studied as regards the complete oxidative sulafte-reducing.During studying the effect of the alkalinity, choosed the lactic acid as carbon resource, to maintain the influent COD 4000mg/L, sodium(SO42-) as electron acceptor, SO42- concentration was maintained at 2000mg/L, so COD/SO42- were maintained at 2:1. During the first phase, The reactor has started up for 43 days, alkalinity is about 500mg/L. The removed effectiveness of sulfate was only 10% to 25%; During the second phase, The reactor has started up for 35 days, alkalinity is about 1000mg/L The removed effectiveness of sulfate was over to 70%; During the third phase, The reactor has started up for 43 days, alkalinity is about 2000mg/L. The removed effectiveness of sulfate was up to 85%. The results showed that:the increase of alkalinity could effectively improve the removal rate of sulfate. There is a big change in microbial as the alkalinity of the changing function of the reactor, the results showed that:in the late of the reactor, the dominant species was Veillonella sp. S101 Candidatus Pelagibacter ubique,Desulfomicrobium baculatum,Desulfovibrio sp. H1,Desulfomicrobium baculatum. The enrichment of these microbes can effective restore the sulfate at higher alkalinity. In which the permanent populations was Alkaliflexus imshenetskii,Thauera terpenica,Sulfuricurvum kujiense,Desulfomicrobium norvegicum. Alkalinity adjustment had little effect on it.In order to discuss the influence of the acetic acid to the sulfate in reducing process as well as the corresponding community dynamics, the reactor is stable when lactate as the carbon source, removed effectiveness of sulfate was up to 80%. When adding acid, the sulfate removal rate was to 10% instantly, then gradually increased to 30%. The results showed that adding acetic acid to the reactor can generate significant fluctuations in operating the reactor. Technical analysis by PCR-DGGE microbial community dynamics found that the functional microbial changed as the carbon source changes. Specific species (Desulfomicrobium escambiense,Bacillus ginsengihumi,Arcobacter butzleri) occurred when added acetic acid to the reactor. In the late of the reactor operation, the species pirochaetes bacterium SA-8 was enriched. Results shows that the population may be able to use acetic acid as substrate to restore the acid sulfate wastewater. Resident species was Cloacibacterium normanense,Arcobacter cibarius,Desulfovibrio intestinalis,Propionicicella superfundia. They all belongs to anaerobic microorganisms which present in various anaerobic environments, some species can take advantage of a wide range of substrate to fermentation acid.
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