Study Of The Purification Process Optimization, Molecular Modification And Application Of Anthocyanin From Pingyin Roses

Anthocyanins are responsible for the blue, the purple, and the red hues, which play a great role in plant physiology as well as possible health effects. Rose(Rosa Ragusa Thumb) is part of the genus Rosa, it grows all over the country and is one part of ten traditional flowers, the flowers of Rose are possessed of High edible and medicinal value,it is generally used for extraction of essential oil, the extraction of anthocyanins from the rose residue after extraction of essential oil can solve the problem of the residue of rose essential oil factory and obtain the natural pigment having safety and health function. The main contents of this thesis are as follows.(1) Basic on the pre-experiment, we find that AB-8 is the best of the resin through the static adsorption and adsorption experiments, then we study the static adsorption and adsorption conditions; On this basis, we study the dynamic adsorption and desorption of the AB-8 resin and the Response Surface Methodology, As a result, the best absorption technology of optimization of anthocyanins from rose was as follows: the concentration of anthocyanins 15.63mg/L, pH 1.92, the current velocity 7.19BV/L, Under such conditions, the absorption amount could be up to 3.42mg; the best desorption technology of optimization of anthocyanins from rose was as follows: the concentration of ethanol 15.63mg/L, pH 1.1, the current velocity of eluent2.72BV/h, the amount of eluants 7.19BV/L, Under such conditions, the desorption rate could be up to 99.19%.(2) Anthocyanins react with Acyl donor (Lauric acid, Acrylic acid, coumaric acid, malic acid, ferulic acid, citric acid and malonic) catalyzing with Novozyme 435 in the non-aqueous environment. The result is verified by liquidoid and IR comparing with the experimental group and non-enzyme control group, In sum, it is deduced that the acylation reaction occurred in the experimental group with lauric acid and acetic acid as the acyl donor, and the acyl conversion rate is worked out.(3) Through single factor experiment, the better level was acquired, on this basis, we select the significant factors through the Plackett-Burman experiment. The significant factors are Shear rate, Solid content ratio and Inlet temperature, and the influence sequence of the significant factors is: Shear rate>Solid content ratio >Inlet temperature. Then by SAS, we build the model with the response value and various factors. the best technologcial conditions were as follows: Shear rate: 13000rpm, Shear time: 1min, Inlet temperature: 161℃, Outlet temperature: 80℃,Core/ Shear ratio: 3.82%, Maltodextrin/βcyclodextrin ratio: 2, Gum Arabic/Solid content ratio: 7.5%, Solid content ratio: 25.57%, then, we study the structure and property of the microcapsule obtained as the best technologcial conditions.