The Screening And Degradation Of Degradation Bacterium In The Crude Oil Polluted Soil By Bioremediation

In recent years, because of the accelerating development of oil industry and the nervous about per capita land, soil pollution problem becomes great harm. It gradually appears on the damage of soil and soil oil pollution caused by pollution and ecological environment, ecological, crop of pollution area and negative health effects. The ground oil pollution of soil environmental caused by oil production and the development process, which once is ignoring, becomes the attention of certain.This article takes the soil by ground oil pollution from oilfield, gets high efficient degradation bacteria on its degradation performance testing. After screening again, the degradation rates of HXB1 which after training 1 week, four weeks.12 weeks at last run up to respectively 44.1%,53.3%,57.6%. are higher than the others'in the same period. Thus ultimately chooses HXB1 as the high efficiency degradation bacterium. The results of its growth rule and optimal conditions for growing are that:the best training time is 16h;pH always in approximately 7 fluctuations. The result of pH is same with the soil pH. Conductivity maximum growth has appeared earlier than the maximum 6h:injection time for training 16h order. According to the growth of degrading bacteria optimum conditions do orthogonal experiment, it obtains the best influence factors for:temperature 20℃,pH for 7. petroleum concentration 6g·L-1, injtction quantity 100ml·L-1, N:P for 1:20, speed for 160r·min-1 The six factors by poor size relations inoculation quantity analysis shows that the biggest influence on degradation effects is injtction quantity, followed by temperature, and the rest of the factors on the effects of degradation are smaller.Crude oil with the microbial degradation basic pseudo-first-order reaction kinetics equation. The degradation of the 7d is better than that of 28d in primary dynamical model of degradation degradation dynamics model (primary correlation coefficient (R2(7d)> R2 28d)), namely continuous degradation bacterium degradation of 7d has better effect than that of 28d. Therefore, continuous long time in soil degradation crude oil pollutant should consider the 7d for a degradation cycle, timely supplements nutrient and degradation bacterium:later, should consider the screening degradation of pollutants more advantageous to lasting degradation bacterium.Using one-dimensional soil column realize the degradation simulation experiments in the soil. Two kinds of soil column get that the degradation rate of ventilation is better than stuffy degradation rate. Transverse one-dimensional soil column airless, degradation rate to note bacteria, and. on the mouth highest center, from side to side, diminishing Ventilated, note bacteria mouth and the note intake proximal sampling mouth degradation rate were increased, remote sampling mouth with airless degradation rate when similar degradation rate. Longitudinal one-dimensional soil column airless, degradation rate to note bacteria mouth proximal supreme, other sampling mouth degradation rate decline one by one; ventilated, the corresponding sampling mouth in soil samples than not ventilation degradation rate when both degradation rate is high. Soil column from two different ventilation note bacteria point, ventilation distance is close, ventilation is still can be assisted microbial degradation of oil, and promote. Far apart ventilation point 1-m control). ventilation (effects are negligible, oil degradation caused mainly by microbial degradation: ventilation, microbial degradation of itself more effects of ventilation degradation function.After inspection of the restoration of soil, it does have biosurfactant produced. The toxicity testing of repaired oil is consistent with the results analysised by statistical test:the results of plant:oil soil of germination rate, fresh weight of root length, stem length, significantly influence, but no significant influence of width.