Study The Submerged Fermentation And The Extract Mycelia Polysaccharide Of Tricholoma Mongolium

Tricholoma Mongolium belongs to Basidimycetes, Basidiomycetes, Agaricales, Tricholomataceae, Tricholoma, which contained a variety of active substances with high nutritional and medicinal value. Using the liquid fermentation of Tricholoma Mongolium not only obtained mount of growth mycelia, extracellular and intracellular polysaccharide, and other active substances, but also made a reasonable development and utilization resources, because its domestication and manned planting was difficult and lacks of substantive breakthrough, as well as its natural habitats were destroyed to result in its wild resource reducing sharply.The main contents and conclusions are listed as follows:1. The paper took Tricholoma Mongolium KM-1 strain as the object of studies. The suitable fermentation medium was optimized by investigations on the effects of different nutritional factors on the production of polysaccharide from KM-1 strain by submerged culture. The results showed that Tricholoma Mongolium KM-1 strain can utilize most of carbon sources and organic nitrogen sources, and the optimum medium formula for shake-flask fermentation was determined as follows: yeast extract 20g/L, glucose 30g/L, KH₂PO₄0.3%, MgSO₄0.3%, which the production of intracellular and extracellular polysaccharides were1.067g/L,0.985g/L,respectively.2. In optimum fermentation medium basis, By single-factor experiments, pH values was determined at 6.5 and the other factors (temperature, inoculation amount, rotation speed) were chosen to further optimum fermentation conditions using orthogonal test. The orthogonal test results showed the optimum fermentation conditions contained 25°C temperature, 12%inoculation size and 180r/min rotation speed, which the maximum intracellular and extracellular polysaccharide production were 1.187g/L and 1.073g/L, respectively.3. Using single-factor test and response surface analysis optimized the hot-water extracted conditions of mycelia polysaccharide. The relation model between the key factors, extracted temperature, extracted time and liquid-solid ratio, and the extracted rate of mycelia polysaccharide wassimulated by a quadratic polynomial:Y=-58.69+1.27X₁+4.75X₂+0.42X₃-3.00E-003X₁X₂+1.00E-003X₁X₃+3.00E-003 X₂X₃-7.58E-003X₁²-1.05X₂²-8.07E-003X₃²The correlation between the true value and the predictive value for the formula was 95.1%. The suitable extracted process conditions for the highest extracted rate of mycelia polysaccharide were as follows: extracted temperature 85.38°C, extracted time 2.18h, liquid-solid ratio 1:31.78g/mL, and alcohol volume 3 times of extracted solution, which the rate of mycelium polysaccharide was 7.23%.