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Bacteria Screening And Experimental Study Of HNS Production Wastewater Treatment

Posted on:2012-04-13Degree:MasterType:Thesis
Country:ChinaCandidate:M WangFull Text:PDF
GTID:2131330335478220Subject:Environmental Engineering
Abstract/Summary:PDF Full Text Request
HNS is a new kind of high-energy insensitive explosive and its application prospect is broad. A set of complex organic synthesis process of HNS will bring large highly toxic and hard-degradation organic wastewater. The HNS production wastewater has characteristics of high content COD, high toxicity and dark color. In this paper, low cost, high efficiency and no secondary pollution method of microorganisms is chased to treat HNS wastewater.This paper inoculating culture microorganism through soil polluted by HNS production wastewater and the sewage of North University of China, 28 strains bacteria with tolerability to HNS production wastewater is obtained. Using these 28 strains of bacteria to HNS production wastewater (diluted 10 times) with flocculation and immobilized treatment. In the immobilized experimental process, an aerobic microbe Bacteria WH3 is got from the North University of China life sewage which has ideal treatment effect to the HNS production wastewater. Through the investigation of wastewater treatment effect, the best culture conditions of Bacteria WH3 are: the culture temperature is 34℃, medium pH is approximately 7 (neutral or partial acid) and training time is 60h (The logarithm period of bacteria growth). Major and minor order of various factors to wastewater treatment effect: training time > medium pH > culture temperature. Considering the treatment effect and practical operation problems, determining optimal treatment conditions are: dosing quantity of bacterium fluid is 40mL, treatment temperature is 20℃and the treatment time is 5d. Major and minor order of various factors to wastewater treatment effect: the treatment time > dosing quantity of bacterium fluid > treatment temperature. Under the certain conditions, replacing the bacteria in 5d, after three cycles, the COD of wastewater eventually decreased to 3520mg·L-1 from 31200mg·L-1 and the removal is 88.7%. The chromaticity decreased to 100 times from 3750 times, and the removal rate is 97.3%. Meanwhile the pH value of wastewater increased to 6.Through simple bacteria identification experiment and observation of appearance characteristics, preliminarily determining Bacteria WH3 belong to acinetobacter. Based on the analysis of the experimental phenomenon and results, the treatment process of Bacteria WH3 to the HNS wastewater has three approaches: the adsorption effect of immobilized materials, the flocculation effect of oozy bacteria and degradation of bacteria metabolic activities to organic compounds. Through research of this paper, an ideal effect was made by using a directional advantage microorganism to treat HNS production wastewater, and it provides experiment and application foundations to treat HNS production wastewater and other explosives production wastewater by using microbial treatments.
Keywords/Search Tags:HNS production wastewater, microorganisms, immobilized processing, culture conditions, treatment conditions
PDF Full Text Request
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