Study Of Surafctant Assisted Extraction And Emulsion Separation Polysaccharides Compounds From Polygonatum

Polysaccharides compounds play important roles in physiogical and pathological effects. It is closely related to the maintenance of biogical function. Studies found that animals and plants polysaccharides have effects of anti-tumor, hypoglycemic, anti-inflammatory, anti-virus, anti-aging, anti-coagulation and so on, and have less side effects on the body. This paper proposed a new polysaccharide extraction method, a variety of surface active agents were used in extracting polysaccharide compounds from polygonatum, proposed double emulsion method to further extract polygonatum polysaccharide from liquid, opening up a new approach of extracting polysaccharide compounds from the liquid.The main research contents and results of the paper are summarized as follows:1. Two extraction process of polygonatum polysaccharides with hot water and surfactants assisting were studied and compared. In this project, the single factor testes were processed to examine the effects of concentrationof surface active agents, extraction temperature, extraction time, ratio of solid to liquid (V/W) and the times of extracting on the yield of polygonatum polysaccharide respectively. On the basis of single factor testes, the extraction conditions were optimized by response surface methodology, the interaction between extraction temperature, extraction time and ratio of solid to liquid (V/W) on the yield of polygonatum polysaccharide were examined, and results were:extracting temperature 92.04℃, extracting time 1.61 h, and ratio of 0.6% sodium dodecyl benzene sulfonate (DBS) solution to raw material 19.93(V/W), under these conditions, a polysaccharides yield (11.10%) was predicted.2. In the separating process of high active polysaccharide by foam fractionation, effects of content of DBS and gas flow on the isolation rate of polygonatum polygonatum were tested, results of the study showed that when the DBS surfactant content was 300 mg·L-1 and the nitrogen flow rate was 150 mL·min-1, isolation rate of polygonatum polysaccharide reached the highest values, were 84% and 83% respectively;3. Polygonatum polysaccharides were further separated by double emulsion of oil in water (O/W) and water in oil (W/O) method mixed with organic solvents with different polar values. The effects of polar values of organic solvents on the extraction rate of polysaccharides were tested, and results showed that extraction rate of polysaccharides got higher and higher as the polarity of organic solvents become smaller.