Studies On Extraction And Purification Process And Anti-inflammatory And Analgesic Effect Of Total Flavonoids From Chinese Starjasmine Stem

ObjectiveTo study the extraction and purification process of total flavonoids from Chinese Starjasmine Stem and anti-inflammatory and analgesic effect of total flavonoids in the best condition of extraction and purification process.MethodsThe single factor test and the orthogonal test were taken to study the best extraction conditions of total flavonoids from Chinese Starjasmine Stem by microwave-assisted method and ultrasonic wave cooperated method through measuring the extraction rate of the total flavonoids by UV spectrophotometry and using statistical Methods for analysis of experimental results. Macroporous adsorptive resins was used in puriting total flavonoids from Chinese Starjasmine Stem for confirming the optimal purification resins and the best purification process through the static-dynamic adsorption and desorption experiments and purpification experiment. The anti-inflammatory effect of total flavonoids from Chinese Starjasmine Stem was researched using ear edema induced by xylene and paw edema caused by carrageen; and analgesic effects of Chinese Starjasmine Stem were observed in pain models of mice induced by acetic acid or hot plate. ResultsThe extraction rate of the total flavonoids from Chinese Starjasmine Stem using the best extraction conditions were higher than the rate of using traditional method by microwave-assisted method and ultrasonic wave cooperated method and could significantly shorten the extraction time. The purity of total flavonoids could reach 86.43% in the best condition of extraction and purification process. The total flavonoids has inhibitory effect on the swelling of mouse ear piece and rat paw edema. The total flavonoids from Chinese Starjasmine Stem have analgesic effects on the two pain models.ConclusionsThe method of microwave and ultrasonic extraction in the best condition was feasible. Absorbent resin could effictively increase the purity of total flavonoids. The puritied total flavonoids from Chinese Starjasmine Stem have anti-inflammatory and analgesic effects.