Synthesis And Applications Of Water-soluble Porphyrins

As the depth study of porphyrins in clinical diagnosis, biological analysis, environmental monitoring and other fields,various synthetic water-soluble porphyrins had been extensively studyed. Especially, in the respects of the analysis and detection of matters in aqueous, the special structures and properties of water-soluble porphyrins make them perfect model compounds for sensor. In this report, we study the applications of water-soluble porphyrin as a fluorescence sensor in biological and environmental fields.In the second chapter of this paper, we were based on the different fluorescence response of water-soluble porphyrin H2TEHPPS to Fe2+ and Fe3+, designed a fluorescent sensor for the detection of H2O2 . According to experimental data, we found that a certain concentration of Fe2+ and a large amount of H2O2 alone in the presence of the H2TEHPPS fluorescence has little influence; in Fe2+ solution once hydrogen peroxide is present, Fe2+ was quickly oxidized to Fe3+, it will make porphyrins fluorescence quenching. Based on the unique nature of H2TEHPPS, We use hydrogen peroxide on oxidation of Fe2+, designed based on highly sensitive porphyrin fluorescence quenching hydrogen peroxide sensor. In the range of 0-8μM of H2O2, we have established a system of linear equations, the ratio of fluorescence intensity of F0 / F for the vertical to [H2O2] is horizontal, R2 = 0.995. H2O2 detection limit is 1.3×10-7 M. Currently, most hydrogen peroxide sensor detection limit is usually about 10-6 M. In comparison, the development of our hydrogen peroxide sensor has higher sensitivity.As the porphyrin H2TEHPPS had a highly sensitive detection of H2O2, we use this feature of H2TEHPPS for monitoring glucose in the glucose oxidase reaction under process, which was mainly due to glucose in glucose oxidase to catalyze the oxidation of glucose lactone, while the release part of the hydrogen peroxide, which can quickly Fe2+ oxidation, leading to porphyrin quenching. According to H2TEHPPS fluorescence decrease can be achieved for the quantitative monitoring of glucose oxidation process. Glucose detection limit was 3.2×10-7 M. In the third Chapter of this paper, we investigated porphyrin H2TEHPPS as the pH sensor is feasible. We study fluorescence and UV absorption of the porphyrin H2TEHPPS under different pH, while common anions and cations on the H2TEHPPS interference were determined and analyzed. The results showed that porphyrin H2TEHPPS had a very sensitve response to the changes of pH, there will be a very sensitive response, and has good resistance to anions and cations interference. Therefore, porphyrin H2TEHPPS can be used as highly sensitive pH sensors used in biomedical, environmental testing.In the fourth Chapter of this paper,we investigated the bingding modes of porphyrin comple (NPh)TMPy and HS-DNA,and found that the (NPh)TMPy could be a promising sensor for nucleic acids.First,we synthesised a soluble porphyrin (NPh)TMPy and charactered with NMR and MS.Then,we studied the interaction of UV-vis spectra, viscometry measurements ,circular dichroism (CD) spectra had been used to study the interactions between porphyrins and HS-DNA ,the results indicated that the binding mode of (NPh)TMPy bound to HS-DNA inclined to intercalation binding.Fluorescence spectra demonstrated that (NPh)TMPy had high sensitivety to HS-DNA in low concenretion.All those features formed the foundation for (NPh)TMPy as a nucleic acids sensor.