| Red tide has become recongnized as marine disasters of the word, severely damaged the marine ecosystem, restricted economic development, affecting the sustainable development of oceans, which has been widespread concern. At present, the prevention and treatment of red tide is basically focusing on prevention, and the treatment method of red tide is still only at the exploratory stage. In addition to traditional physical and chemical methods, microbial dissolution of red tide algae also become an important.Two algae-lysing Actinomycetes, named as B-10 and G-11, were isolated from the soil in Wuhan. Their algicidal effect on Heterosigma akashiwo, Scrippsiella trochoidea, Prorocentrum minimum and Golden algae were tested, and the algicidal mechanisms were discussed. The two Actinomycetes were then identified according to their characteristics in morphology, physiological and biochemical properties and 16S rDNA sequences. Actinomycetes B-10 and G-11 could remove 90% and 99.44% of H. akashiwo at the inoculum dose of 1%(V/V) in 5 days, respectively; B-10 and G-11 could also remove P. minimum and G algae; the algicidal abilities of the B-10 and G-11 were stable in the culture of 5 generations; both B-10 and G-11 lysed H. akashiwo by releasing algicidal substances, which were susceptible to the temperature higher than 100℃; B-10 belongs to Streptomyces microflavus and G-11 belongs to Streptomyces variabilis.To explore the structure and properties of lytic substance of actinomycetes G-11, use of dialysis, ethanol precipitation, organic solvent extraction and other testing methods to study its physical and chemical properties. The results show that algicidal substance of actinomycetes B-11 may be one or more lipophilic substances, and its Quality points greater than 3.5KD.
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