| Phenyllactic acid (PLA), which is produced by some strains of lactic acid bacteria, is one of importance agent in food industry. It plays an important role in preservation and prolonger shelf life of food and feed. This study investigated the method for extracting and purifying PLA from fermented solution, both static adsorption and dynamic adsorption being studied.6 types of resin (301,315,335,345, HZD-9, HZ-2) were testing for adsorption and desorption capacity. Among these,301 resin was the best one and was chosen for further steps. The fermented was first preparation with active carbon and heating at 80℃for decolorizing, and then prepared solution was introduced to resin 301 for adsorption. Second, HCl was utilized to dissociate PLA from resin; pH of the outlet solution was then adjusted before extraction procedure with ethyl acetate. Finally, extracted solution was condensed with the aid of rotary evaporator for crystallizing. The HPLC mobile phage was modified as 50:50 of solvent A-Triflouroacetic acid (0.05%)/water and B-Triflouroacetic acid (0.05%)/methanol and flow rate of 1 ml/min. The retention time of PLA in this HPLC condition was about 5 minutes. The adsorption of PLA on 301 resin was well fix with Langmuir equation and the coefficient (R2) of relative equation was over 0.99, and free energy change (AG) of adsorption process was negative. The recovery rate of desorption was 87.31% and the purity of the product was 95.07%.
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