Construction Of Efficient Phenol Degradation Microbial Agent And Its Bioaugmentation Of Phenolic Wastewater Treatment Sysytem

The source of phenolic waste water is extensive and the compositions are complicated, such as pharmaceutical, coking, chemical and dying wastewatwer. The existence of high concentration of phenolics will inhibit the growth of the microorganism and brings more difficulty in biological treatment, which has become a difficult problem in domestic and international wastewater treatment field and is urgent to be solved currently. To solve the problem of inhibitory effect of high concentration of phenolics on traditional biological treatment system, the objectives of this paper are to explore of bioaugmentation treatment systems, which are capable of degrading phenolics, study on the characteristics of treatment systems under different conditions and analyze the community structure during treatment process by molecular biology technology, such as PCR-DGGE. The experimental results will clarify the relationship between the microstructure and macrofunction of treatment system and provide a theoretical and technical supports for the biological treatment of phenolichyper-saline wastewater.Alcaligenes sp.TF1, Acinetobacter sp. TF2 could utilize phenol as sole carbon source , which was identified on the basis of physiological and biochemical tests and polyphasic-taxonomy technique. Studing on physiological-biochemical characteristic of TF1 and TF2, both of them have strong ability of phenol degradation , The ability of phenol degradation of TF2 is always higher than the TF1's , whatever Changes in growth conditions and circumstances. The optimum cultural conditions of TF1 obtained according to the single factor experiment and the orthogonal experiment was:25℃, pH7, Initial phenol concentration 100mg/L, rpm140, Initial inoculation was 10%; The optimum cultural conditions of TF2 was : 25℃, pH9, Initial phenol concentration 100mg/L, rpm100, Initial inoculation was 2%. The best mixture ratio Strains of TF1 and TF2 is 2.33:1.Analyzeing the effect of bioaugmentation microbial inoculums, the data show that regardless of whether the temperature and phenol concentration changes, bioaugmentation microbial inoculums On the treatment effect of phenol and COD have achieved the highest level. The types of organic compounds in draining water has been reduced to 32 species from 68 species. Long chain alkanes in the water treating by the bioaugmentation microbial inoculums was significant decline, benzene, esters, aldehydes and ketones were removed almost, the number of organic compounds also reduced by about 50%.Analyzing the community structure during treatment process by molecular biology technology, such as PCR-DGGE, shows that regardless of whether temperature and phenol concentration changes, bioaugmentation microbial inoculums system has reached the highest abundance leves. Compared with those systems, which did't treat by bioaugmentation microbial inoculums, the microbial species composition has changed significantly. Comprehensive analysis with the degradation of phenol,COD and the types of organic matter in draining water, show the effect by bioaugmentation is significant.