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The Bioagumentation Efficiency Of Microbial Agent In MBR Process For Domestic Wastewater

Posted on:2020-02-09Degree:MasterType:Thesis
Country:ChinaCandidate:Y W SunFull Text:PDF
GTID:2381330611998680Subject:Marine science
Abstract/Summary:PDF Full Text Request
Water pollution is still one of the ecological problems to be solved urgently.The traditional sewage technologies can not fully meet the requirements of people with high water quality,and the inoculation of microbial agents provides a new method to improve the sewage treatment effect.However,many microbial agents are prone to loss,weak impact resistance,and poor degradation.This project will use yeast composite microbial agent to strengthen the Membrane Bioreactor(MBR)system to treat simulated wastewater through the combination of bioaugmentation and microbial immobilization.It is expected to provide a theoretical basis for the combination of the two technologies and also provide a method for the application of the yeast agent.In this study,the ordinary MBR was used as a control to compare the degradation effects of pollutants,and the effect of different dosage forms of microbial agent on the MBR process was studied.Preliminary study on the mechanism of bioaugmentation in MBR process by high-throughput sequencingIn the optimal operating parameters,the effect of bioaugmentation in MBR was investigated by seeding free microbial agent.It was found that the degradation effects of COD,ammonia nitrogen and TN in the experimental group and the control group were slightly different,and the effect of bioaugmentation was not ideal.The polypropylene suspension carrier was selected to form biofilm consist of the sludge and the microbial agent,and the biofilm was applied to the MBR system.The treatment effects were explored under normal load and shock load operating conditions.Under normal load,the average ammonia degradation rate of the experimental MBR system was 89.43% in the first week,and in the control group was82.86%.With the operation of the reactor,the ammonia degradation effect was not obvious.The average degradation rate of TN in the experimental group was 15.72%higher than that in the control group,while the average degradation rate of COD in the two MBR systems was not significantly different.Under high load operating conditions,the experimental group did not show strong impact resistance.Based on high-throughput sequencing,it was found that the relative abundance of the dominant fungal community of the experimental group was consistent with the trend of ammonia nitrogen in the system,indicating that the microbial agent played a key role in the enhancement of ammonia nitrogen and TN.Preliminary judgments of thepresence of Proteobacteria and Bacteroidetes resulted in high COD degradation of both systems.Inoculation of the optimized embedded microbial agent into the MBR system has a better treatment effect.Under normal load,the ammonia average degradation rates in the experimental and control systems were 93.36% and 83.83%,respectively,and the TN average degradation rates were 65.36% and 37.37%,respectively.However,The COD degradation effect of the two systems was similar.Under shock load,the experimental group showed stronger impact load.Compared with the direct inoculation of free microbial agent,the method of embedding immobilization is more conducive to the bio-enhancement of the microbial agent and has certain impact resistance.The microbial agent was adsorbed by suspension carriers and embedded all have better bio-enhancement degradation effects and can improve the impact resistance of the system.This paper provides some methods for the application of microbial agents,especially yeast agents,in wastewater treatment.
Keywords/Search Tags:bioaugmentation, immobilization, microbial community structure, microbial agent
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