Analysis Of Aromatic Compounds And Carbamate Pesticide In Water By Liquid Chromatography

With the development of economy and society, the water pollution has been the main environment problem and has been given more attention to the public. The pollution of aromatic compounds and pesticide residues are two important aspects, so it is necessary to monitor these two kinds of environmental pollutant. In the fisrt chapter, we summarized the characteristics and hazard of aromatic compounds and pesticide residues, and introduced the main analysis methods to these two kinds of pollutant. In the second chapter, new methods for separation and determination of the aromatic compounds by HPLC were established. Based on salting out phase separation microextraction and HPLC, a novel mothod was used for the determination of substituted benzenes in water samples. The types of extractant and inorganic salt, the extactant volume and saltinity were investigated. The methodology exhibited good linearity and the detection limits were 0.07μg/L for o-chlorophenol, 0.2μg/L for chlorobenzene and 9.0μg/L for ethylbenzene, respectively. The average recoveries and relative standard deviations were 71.7%-93.0% and 1.2%-5.2%, respectively. A simple and sensitive analytical method using microextraction flask coupled to HPLC has been developed for the determination of polycyclic aromatic hydrocarbons (PAHs) and bisphenol A (BPA) in seawater. The impact of extractant, extractant volume, extraction time, salinity and pH value was investigated. For PAHs, the methodology exhibited good linearity between 0.01 to 50mg/L; the detection limits were 7.0-13.3ng/L; the average recoveries and relative standard deviations were 92.6%-94.5% and 0.9%-1.8%, respectively. For BPA, the methodology exhibited good linearity between 0.01 to 10 mg/L; the detection limit obtained by this method was 0.03μg/L; the recoveries of simulated seawater for 1.0 and 10.0μg/L were 86.5% and 83.1% with the relative standard deviations below 6%. In the last chapter, the method using salting out phase separation microextraction and HPLC was applied again for the determination of carbamate insecticides residues from water. Extraction solvent was iso-butyl alcohol and salting out agent was ammonium sulfate. A C18 column was used for separation with detection wavelength at 220nm; the mobile phase was a mixture of methanol and water (the ratio of volume was 53: 47). The detection limits were 0.4-5.7μg/L. The average recovery of pesticides ranged from 78.6% to 89.8% with the relative standard deviations below 5.0%.