A New Strategy For Molecular Imprinting And Its Application In Metal Ions And Western Blot

Molecular imprinting technology(MIT) simulates the interaction between enzyme and substrate of the nature, and creates molecularly imprinted polymers(MIPs) with tailor-made binding sites complementary to the template molecules in shape, size and functional groups. Caused by its rapid development and the new strategy for continual discovery and application, imprinting technology becomes the hot spots. In the analysis of many targets, ion imprinting and protein imprinting have become two challenges of imprinting technology due to some characteristics of the templates. In this paper, two novel strategies for above imprinting were developed, and the detail contents are as follows:1. Synthesis and application of multi-ion imprinted polymer based on dithizone chelationA novel general IIPs strategy for simultaneous preconcentration and removal of four heavy metal ions was proposed for the first time, based on dithizone chelation. The multi-ion imprinted polymers(MIIPs) embedded in a sol–gel matrix were prepared by using Hg(II), Cd(II), Ni(II) and Cu(II) as templates and 3-aminopropyltriethoxysilane as a functional monomer, and the possible synergy mechanism was explored between dithizone coordination chemistry and multi-ion imprinting. The resultant MIIPs showed high binding capacity and fast dynamics, and the adsorption processes obeyed Langmuir isotherm and pseudo-second-order dynamic models. The MIIPs displayed excellent selectivity toward the four target ions particularly over Pb2+, Zn2+and Co2+ with selective coefficients of 6.8–16.9, as well as high anti-interference ability confronting with common co-present various ions. Consequently, the MIIPs were used as solid-phase extraction(SPE) sorbents for preconcentration of trace Hg(II), Cd(II), Ni(II) and Cu(II), presenting high detectability up to 6.0–22.5 ng L-1 and satisfactory recoveries ranging from 94.7–110.2% in seawater samples. Multiple ions as a complex template, MIIPs are prepared efficiently via a simple and relatively universal ligand. Certain kinds of ions can be recognized and enriched simultaneously, which has a promising application prospect.2. Synthesis and application of thermosensitive molecular imprinted nanosensorBased on free radical polymerization, the thermosensitive imprinted nanosensor was prepared using phycocyanin as template via a simple and facile approach. It can controlled release the protein followed by the temperature, recognize and fluorescence detect phycocyanin efficiently by virtue of QDs with the TGA-GSH co-modification. Preparation and possible mechanism of MIPs, the characterization of the sensor, intelligent response to the temperature, binding performances, molecular selectivity of the receptor, analytical performance and application were studied systematically. It presented a satisfactory linearity in the range of 0.01-1.8 μM and a good detection limit of 10.3 n M. Moreover, recoveries ranging from 92.0%-106.8% were attained for spiked seawater samples. The study provided a facile, fast and intelligent way for identification analysis of trace proteins from complex water matrices, and further pushed forward protein imprinting and stimuli-responsive molecular imprinting research.