Viral Molecularly Imprinted Fluorescent Sensor With High Sensitivity For Simultaneous Detection Of Multiple Hepatitis Viruses

Because of highly contagious nature and the absence of immediate and efficient control systems,viruses have brought huge losses and suffering to humans,hence,accurate diagnosis and detection of the virus is essential and urgent.Especially for similar viruses,their clinical manifestations are similar,which makes it difficult to diagnose accurately.Therefore,the simultaneous detection of two or more viruses is of great significance.Although molecular imprinting sensors have been widely studied and applied in the detection of viruses,the simultaneous detection of two viruses by molecular imprinting still faces great challenges,because of their similar structures or the same functional groups,which causes non-neglected interference.Meanwhile,there is great improvement room in the selectivity and sensitivity of viral molecular imprinting sensors need to be improved,which makes them limited in practical applications.Therefore,a molecular imprinting sensor for simultaneous detection of virus was constructed.In addition,we are committed to improve the comprehensive detection performance of the virus imprinting sensor in our works,.1. A visual molecular imprinting fluorescence sensor for the simultaneous detection of hepatitis A virus and hepatitis B virus was developed.Firstly,green Cd Te quantum dots?G-Cd Te?and red Cd Te quantum dots?R-Cd Te?were selected as the output signal source of hepatitis A virus?HAV?and hepatitis B virus?HBV?,respectively.A layer of silicon dioxide was wrapped around the QDs by tetraethyl orthosilicate?TEOS?hydrolysis to form aggregates of multiple quantum dots,which was used as the imprinting carrier,zinc acrylate was selected as the functional monomer,and two viruses were used as templates of imprinting particles?G-MIPs and R-MIPs?,which showed selective adsorption for HAV and HBV respectively.The introduction of N-isopropylacrylamide as both a temperature-sensitive monomer and a hydrophilic monomer can not only endow the sensor temperature-sensitivity,but also reduce non-specific adsorption and improve the selectivity.Then the molecular imprinting fluorescent sensor that detects HAV and HBV simultaneously was flexibly obtained by mixing two imprinted particles at a certain ratio.The detection limits are3.4 pmol/L and 5.3 pmol/L for HAV and HBV,respectively,and the naked eye can be realized under ultraviolet light irradiation due to the to the color changes of green quantum dots and red quantum dots.2. The fluorescent metal organic framework?MIL-101-NH₂?was used as the imprinting carrier,which can provide more imprinting sites.At the same time,it can be used as one of the fluorescence signals of the ratio sensor.The detection linear range and sensitivity were improved and improved due to the advantages of the low background signal of the ratio type fluorescence sensor.In addition,the aptamer of hepatitis B virus was selected as the functional monomer.Since the aptamer can specifically recognize the target molecule and form a dual recognition effect with the imprinting sites of templates,the selectivity of sensor was greatly improved against the target hepatitis B virus,and the imprinting factor reached 5.2 for the first time.Compared with other viral molecular imprinting sensors,the overall performance of the sensor is more satisfactory,increasing the potential of the sensor in practical applications.