Study On Microbial Fermentation Modification Of Rhubarb And

Fermentation is an important method to process traditional Chinese medicine (TCM). Fermentation processing could improve bioactivity, and reduce side effect of original medicinal material. It has more than 1000 years from fermentation medicine first appeared in China. In recent years, more and more people pay their attentions to the fermentation of TCM. In this thesis, nine strains of plant pathogenic fungi were used to ferment the rhizome of Rheum palmatum. And five free anthraquinone in the rhizome of R.palmatum were quantitatively assayed with the proton nuclear magnetic resomance (1H NMR). Additionally, twelve plant pathogenic fungi were used to study the fermentation of the flowers of Magnolia denudata. This thesis describes the fermentation processing of above two TCMs in three chapters.The first chapter is about the development process, experimental principle, optimizing parameters and application progress of the quantitative 1H NMR in different scientific research field.In the second chapter, using the quantitative 1H NMR to determine the contents of five free anthraquinones (rhein, emodin, chrysophanol, aloe-emodin, and emodin monomethyl ether) was described. Acetone-d6 was selected to be optimum solvent after screening the separation effect of the different deuterated reagent. By using 1H NMR, above five free anthraquinones in the rhizome of R. palmatum were analyzed qualitatively and quantitatively. It is feasible, accurate and stable to use 1H NMR to analyze five free anthraquinons in the rhizome of R.palmatum. This work is meaningful to the quality control of R. palmatum. Nine plant pathogenic fungi (Trichoderma sp, Isaria sp, Botrytis cinerea, Fusarium avenaceum, Fusarium oxysporum, Helminthosporium maydis, Trichophyton verrucosum, Penicillium islandicum and Pyricularia grisea) were used to undertake fermentative experiment of R. palmatum. It’s found that the antibacterial activityof Trichophyton verrucosum fermented R. palmatum was increased abviously. And its total anthraquinone content (TAC) was about three fold higher than original materials, which indicated that T. verrucosum fermentation could facilitate the accumulation of anthraquinone compounds in R. palmatum.The third chapter shows the fermentation of the flowers of M. denudata using twelve plant pathogenic fungi(Fusarium solani, Fusarium avenaceum, Aspergillus tubingensis, Fusarium oxysporum, Myrothecium inundatum, Aspergillus sydowii, Penicillium virgatum, Alternaria alternate, Aspergillus versicolor, Pyrenochaeta inflorescentiae, Acremonium furcatum and Nigrospora oryzae).Basing on TCL and antibacterial activity screening, it’s found that a-terpilenol in the flowers of M. denudata was transformed to oleuropeic acid by the fermentation of A. alternate. The biotransformation of pure a-terpilenol further demonstratedthat oleuropeic acid was transformed from a-terpilenol. This work is meaningful to the biotransformation of monoterpenes.