Study Of The Inactivation Effects And Mechanism Of Pulsed Light On The Typical Microorganisms

With people's living standards improving, requirements on drinking water quality have become more sophisticated. In recent years, climate change, earthquakes, tsunamis and other natural disasters on the global occurred frequently and caused a great impact on source of drinking water, threatened the drinking water safety.World Health Organization (WHO) survey found that more than 60% human disease which caused by the bacterial are infected through drinking water. Safety of drinking water for people, must be drinking water disinfection.The method of drinking water disinfection mainly include the liquid chlorine to disinfect, two oxidize chlorine disinfection, ultraviolet radiation disinfection and ozone to disinfect etc. The development of modern analytical techniques and health toxicological studies, it was discovered that chlorine disinfection disinfect effective, think towards cleaning the aquatic organic matter result disregard, would also born 20 various vice-outcomes, include three bittern acetic acid etc. carcinogen and chlorine disinfection is not effective against Cryptosporidium and cysts; In the ozonation process will produce a carcinogenic effect of bromoform, dibromo acetic acid and bromate and other by-products, and chlorine dioxide and its by-products chlorite animals will have some negative effects of the biological; UV disinfection use low pressure mercury lamp to disinfection in a row, low power, poor penetration of the water layer, while inactivation by UV irradiation viruses and bacteria can be light to restore the damaged tissue itself, to revive the purpose of (light repair), and even repair itself without light (dark cultivation), so its sterilization is very restricted.As a new pulsed light sterilization technology has been proven to have a potential drinking water disinfection effectiveness. However, little research at home and abroad, it was still a blank in china.This study investigated the pulsed light inactivation of Escherichia coli, Staphylococcus aureus and Bacillus subtilis and the effect of influencing factors, combined with transmission electron microscopy (TEM) and scanning electron microscopy (SEM), observed the pulsed light irradiation changes the form and structure of bacteria. The results show that:(1) Pulsed light on the water in Escherichia coli, Staphylococcus aureus and Bacillus subtilis inactivated strong performance. The best voltage to typical pulsed light inactivation of microorganisms is 3000v. Light flashes 12 times Escherichia coli, Staphylococcus aureus, light flashes 14 times, Bacillus subtilis, light flashes 20 times, inactivation rates have reached 5.31 log. Inactivation rate of Escherichia coli and Bacillus subtilis increases with the bio-load.(2) The influence on typical microorganisms with pulsed light sterilization is that Bacillus subtilis