Researches On Key Technology To Improve Resolution Of The Microscopic Imaging

Since the birth of the first microscope, the microscopy imaging technology has been widely applied in the microcosmic field that it covered just like medicine, biology, electronic, military, industry and so on. A lot of novel microscopy imaging technology are coming forth continuously with the application of high-tech. For the fast development of information technology, the research on microscopic imaging has become the important topic all around the world. In this paper, we has completed the embedded research on key-technology to improving transverse resolution and longitudinal resolution of microscopy imaging system, which is oriented at the two kinds of new-style microscopy imaging technology that are digital microscope system and optical coherence tomography (OCT) as research objects.The thesis brings out the conception of transverse and longitudinal resolution of microscope system, following the development survey of microscope imaging technology as the fore-guidance and with the baasis of the theory of confocal scanning optic microscopy as example which is the hot point of microscope field. Based on the deep theoretical research on how to improving transverse resolution and longitudinal resolution of microscopy imaging system,, two methods are given in this thesis, one is about how to improve transverse resolution based on the digital image processing and measurement technology which utilize interference microscope as core of the system, and another is about how to improve longitudinal resolution of OCT experimental system by using self-designed cam-driving longitudinal scanning wheel. The paper encloses the methods giving above and gives out a set of digital microscope system. The corresponding digital image processing software coded on Microsoft Visual C++ platform has been developed, then a deep research on the Fresnel microlens arrays by using the developed system is excuted and the experiment results of improving transverse resolution are achieved in this paper. A set of OCT experimental system which the cam-driving longitude scanning wheel is as the core component is established, by this system, we obtain about 10 micron longitudinal resolution from experiments results on glass sample and so on, comparing with the experiment data from practical eyes-examine OCT system.The research on key-technology about how to improve transverse and longitudinal resolution of this paper has practical value for equipments promotion and widely application in the microscopy field.