Study On Carbonate-Mineralization Microbe

Some bacteria in nature can keep occurring enzyme digestion process with surrounding medium through its own life activity, which, after a long-time accumulation, enable to agglutinate those precipitated loosen classic substances into solid rock. This kind of bacteria is named carbonate-mineralization microbe in this study.Enlightened by the phenomenon, if discovering this carbonate- -mineralization microbe, supplying which with enough and proper conditions of its existence, propagation and activated reaction, accelerating whose enzyme digestion process, and precipitating CaCO3, we can apply this technique that agglutinates lithoid materials within a short time through bacteria to many fields, for instance: to assist great architectural projects and to repair hiatuses in important historical buildings, to agglutinate regenerated concrete and to precipitate, separate heavy metal ions in sewage disposal.According to the pivotal enzyme digestion characteristics of carbonate-mineralization microbe, this study processes large amounts of preresearches on the selection of the bacteria, and eventually purchases bacteria A from oversea conserving center of bacteria, after that, we developed careful research on many aspects, such as its physical and biochemical characteristics, individual growing characteristics, its rules on colony growth and propagate, also its incremental propagate technic, the research proves bacteria A possesses the pivotal enzyme digestion characteristics of carbonate-mineralization microbe and grows and propagates quickly. It belongs to mesophiles whose growing conditions prefer to be PH=8.0, however, it shows vigorous life force still in strong-alkali environment.Based on the research on the physical and biochemical characteristics of bacteria A and its influencing elements of environment, we takes advantages of enzyme digestion process happening in the growth of carbonate-mineralization microbe----bacteria A, and adds Ca 2+ properly, thus orbicular CaCO3 whose diameter is less than 10μm is precipitated successfully, which is proved to be calcite crystalloid through XRD analysis. In addition, the crystal shape of CaCO3 and its granule figure will change to some extent when the environment reaction conditions, such as pH, temperature, substrate,culture, source and consistency of Ca2+, etc.Besides these, the study also develops some alike contrastive experiments by using the artificial distilled vegetable enzyme, favorably some good rhombohedron-shape calcite is obtained, too, which validates that the key to the precipitation and mineralization of CaCO3 is the enzyme digestion characteristics of carbonate-mineralization microbe, and its main conditioner and controller is water-soluble matrix(SM) with rich anionic groups which is contained in the interface of bacterial membrane,the SM constructs the growth of calcite constantly and controls the shapes of CaCO3.