| Mycorrhizal fungi (Arbuscular mycorrhizal fungi, AMF) is a broad and important microbial, through the establishment of mycorrhizal relationship with land plants to affect plant growth and development, and improve plant resistance and productivity. Licorice (Glycyrrhiza uralensis Fisch) is a perennial herbaceous legumes (Leguminosae), it is a valuable resource and medicinal plants. Licorice is facing resource depletion or extinction, because wild licorice serious ecological deterioration and desert areas of excessive excavation.. Licorice has evolved a set of adaptive mechanisms and strategies, in order to adapt the local water deficit and climate characteristics, in the long-term evolution.This paper we have done three tests use mycorrhiza biotechnology by medicinal plants of licorice having resistance as a model plant. Test I:analysis of licorice drought resistance and m status of ycorrhizal microorganisms using typical legume inoculation of mycorrhizal fungi of licorice pot simulation test to understand resiliency and tolerance by drought stress, rehydration after the drought. As the basic research for the licorice plant drought-resistant mechanism, mycorrhiza biotechnology applications, land reclamation, soil conditioner. Test II:add five kinds slurry gradient (high, medium, low, and without) treat weekly by inoculation interaction. Meet application security and improve the yield of licorice to protect the security of the soil for the development of product with biogas and agents matching optimization to provide a theoretical basis. Test â…¢:biogas and fertilizer, different gradient (high, there is none,) interaction test, research biogas interaction with agents, growth and yield conditions licorice, licorice plants on soil nutrient absorption capacity, fertilize of biogas and fertilizer for soil safety and choose the best gradient. The main findings are as follows:1.Verify that the AMF can significantly increase the drought tolerance of licorice. AMF can improve recovery capability after drought and accelerate recovery progress of licorice.18-22week, the phosphorus concentration in the shoots and roots, not only not decreased but is gradually growing after AMF inoculation. AMF inoculation and drought stress improve licorice phosphorus absorption capacity, the phosphorus concentration in the control group (C) is increased after rehydration. Pn, WUE are reduced drought stress, increased after rehydration in the plants with AMF, without AMF inoculation (C) on the contrary, is to reduce the stress, elevated after rehydration. Tr is increased drought stress, rehydration continues to rise with AMF inoculation, but no inoculated AMF control group (C) is the stress increase, decrease after rehydration.2. Research slurry and AMF on plant growth, plant nutrition, safety impact, finally get the optimal ratio of biogas and agents group. Licorice can increase2.5to4times the biomass, chlorophyll content was significantly increased by applying a high amount of biogas and AMF M10. We found that AMF can reduce the risk of slurry applied, AMF interaction energy with biogas has increased soil organic matter content, increased soil phosphorus concentrations, so increasing soil activity.3.Research biogas and agents ratio on plant growth, plant nutrition, soil safety and identify safety biogas fertilizer. In the present study, the mixing gradient of biogas and fertilizer, M10is preferably integrated, licorice can be improved more than3times the biomass, chlorophyll content was increased. And it can improve soil phosphorus concentrations for plant growth, but also to reduce the biogas residue in the soil risk.
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