Identification, Detection And Genetic Diversity Of Colletotrichum Gloeosporioides

Anthracnose is an important pathogenic fungus with a wide range of hosts, especially, which happened in tropical and subtropical regions, and can damage a variety of crops, cash crops, trees and fruits and vegetables to modern agricultural production caused serious economic losses.In this study, the disease samples of anthracnose of Litchi were collected from different tissues, fruit and leaves, of Litchi in six provinces of southern China of Guangdong, Guangxi, Hainan, Fujian, Yunnan, Sichuan. We isolated and obtained116Colletorichum strains from these disease samples, according to morphological characteristics and molecular means of identification. There were114strains of Colletotrichum gloeosporioides by morphological identification, only one was Colletotrichum acutatum, the other one was unknown. By the molecular identification, there were98strains of Colletotrichum gloeosporioides, and only one was Colletotrichum acutatum, the results were consistent with the morphological identification. In addition, the study found that the sexual state of Colletotrichum gloeosporioides, the ascus had eight ascospores.Pathogenicity differentiation tests of Colletorichum strains from different origins were carried out by in vitro inoculcated on young leaves of’feizixiao’Litchi. The results indicated that the pathogenicity of different Colletotrichum strains was distinctly different. The pathogenicity of the Colletotrichum significantly related to its geographical and tissue origin.Morphological characteristics showed a great difference among6Colletotrichum with different pathogenic types. Moreover, it was some obvious correlation between morphological diversity and pathogenicity.100Colletorichum of Litchi in China were divided into ten distinct clusters based on AFLP. The classification of clusters mainly derived from geographical origin of strains, also from pathogenicity and tissue strains.