Study On Host Characteristics Of Bamboo Shoot

Shirara bambusicola P. Hennings is a medicinal fungus growing on the twigs of bamboo plants with the higher value in medicinal use. Along with the discovery of the photosensitive hypocrellin (hypoerellin A and hypocrellin B), Many kinds of active ingredients have more and more received attention in the medicine, food pigment and biological pesticide. S. bambusicola has increasing received recognition in research and application. The natural resources of S. bambusicola have rapidly reduced recently because of the large-scale collection and the habitat destruction. The resources would be in severe danger if there are no any protective measures and availability of sustainable use strategies. It is imminent to protect the resources of S. bambusicola.Almost no spore production under the experimental conditions, to understand S. bambusicola on the host, it was found that the mycelium germination, growth stage, infection and colonization by the microstructure can intuitive understanding interactions between S. bambusicola and host,even learned formation conditions of the stroma of S. bambusicola. This study explores the condition of producing protoplast, obtained by protoplast can be resurrected; When transform the genetic of S. bambusicola. Three kinds of methods of PEG mediated, electrical transformation and Agrobacterium tumefaciens-mediated transformation have used for transform genetic, but unable to get positive transformation. The microscopic observation of S. bambusicola has accurately determined the interactions. Confirm the behavior on host as pathogenic bacteria, the specific conclusions are as follows.(1) To explore the condition of preparation of protoplast, the results show that the highest production of The 5 day old mycelia treated with the mixture of 1.0% Lysing Enzymesand 1.5% Driselase in 0.6mol/L D-Glucose at 29℃ for 2.5h.Resurrection medium choose 0.6mol/L D-Sucrose as the permeability stabilizing agent, and the resurrected rate could be reach 54.8%.(2) Because of S. bambusicola can inhibit the growth of agrobacterium, the agrobacterium tumefaciens- mediated transformation is the low efficiency method. This study also failed to for a positive transformation through the law.(3) This study explores the condition of introducing fluorescent mark material by electro-stimulation and PEG-mediated, efficiency of the transformationsare low.This study also failed to for a positive transformation through this laws.(4) Under the action of fluorescence microscope, the bamboo structure emit greenish yellow light after being excited by blue light, and the S. bambusicola emit red. So it is not good for use the GFP protein as markers to observeS. bambusicola hyphae with the host.(5) The infection structure and source of nutrients have observed by scanning electron microscope observation, it can be preliminarily determined the S. bambusicola as a kind of pathogen parasitic in the bamboo. It is one of the reasons what resulted in the deaths of bamboo.