Principle Of Biochemical Metabolism Of Cysticercus Cellulosae And Mechanism Of Action Of Drugs

By detecting a few biochemical indexes of C.cellulosae in the development and treated by drugs,the article studied principle of biochemical metabolism of C.cellulosae and mechanism of action of drugs and provided a theoretical basis for preventing and treatment of cysticercosis. Three kinds of ATPase and ALP wtiich join absorption and transport of substance, Glc HK. MDH. PK. LDH. LAC and G6PDH in glycometabolism,TG in lopid metabolism,GOT GPT,GDH and UN in amino acid metabolism and XOD and UA in purine metabolism were detected .Activity of HK.. PK MDH. NaKTPase and content of GLc of bladder wall and activity of Ca2TPase of scolex rised with the development of C.collulosae.The results showed that transport of substance,anaerobic glycolysis,aerobic glycotysis,phosphopentose pathway and amino acid metabolism were same as those in other helminths. With the development of C.cellulosae,fiinction of membrane transport,the pathways in which HK catalyzed GLc to metabolize and PEP formed LAC with the catalysis of PK and LDH and the pathway in which the parasite entered into reverse tricarboxylic acid cycle enhanced. The detection results of effects of drugs on biochemical metabolism of C.cellulosae cultured in vitro in different periods showed that NX NX1 and NX2 had some actions on oncospheres,immature and mature C.cellulosae and NX0 had the same effects against biochemical metabolism of C.cellulosae as NX1 did.First activity of MDH and content of GLc decreased and activities of PK and LDH and content of LAC increased. At the later stage ,alI the metabolism systems were effectd and activies of enzymes decreased and metabolite accumulated. The results showed that drugs inhibited the pathway of aerobic glycolysis and anaerobic glycolysis strengthened compensatorily and then the metabolism of glycolysis were inhited,which resulted in exhaustion of energy and death of C.cellulosea. According to the therapeutic effectiveness of dntgs,NX2 was ineffective and NX1 was better than NX0 and NX1 had better treatment agaist immature C.cellulosae than against mature C.cellulosae in vivo. Postgraduate: Gao Wcn xue Major: Basic VeterinaiyAdvisor: Professor U Qingzhang...