Cryopreservation Of Spermatoza Of Marine Economic Animals

In the past few decades, methods for successful cryopreservation of spermatozoa have been developed for domestic animals. And cryopreservation of the spermatozoa of salmonid fishes and oyster has been well studied. But until now only fewer species of marine animal were studied. In the present paper, attempting to develop simple~ reliable and efficient methods for cryopreservation are reported. Cryopreservation of sperm cells of three kinds of shellfish and two kinds of fish was investigated in liquid nitrogen. Motility, fertility and hatchery rate of 49.39, 42.06 and 12.15% were obtained in cryopreservation of the spermatozoa of Chlamys farreri using a cryoprotectants dilution (DMSO5%+sucrose5%) and freezing rate 20扖/min. The volume of samples between 0.6 to lml is suitable for cryopreserving spermatozoa of scallop. Moreover, equilibration at 0C is necessary, and 2 minutes is enough for that. The optimal thawing temperature is 350C in water bath, 20C worst. For sperm of scallop the freezing damage occurred at -30C to -60 C. The nature seawater is good to activate the thawed sperm. The addition of yolk is harmful to hatchery of sallop. The motility of sperm is 42.17% after storaging 55 days in liquid nitrogen (LN2). The optimal motility, fertility and hatchery rate of 41.63, 69.52 and 54.74% were obtained in cryopreservation of the spermatozoa of Mytilus gallopmvincialis Lamarck using a cryoprotectants dilution (DMSO15%+sucrose5%) and freezing rate 50C/min. The better activator solution is the nature sea water. The volume of sample has no correlation with the result. The motility of sperm is 41.8% after storaging 90 days in LN2. The lager freezing damage of sperm of Ruditapes philippinarum (Adams and Reeve) is occures at -30C to -6(YC. The suitable freezing rating and extender dilution is SC/mm and DMSO1O%+sucrose5%. The effects of cryoprotectants is DMSO better than glycerol. The nature sea water is best suitable activator solution. 4 The volume of sample can affect the result of cryopreserving. The maxmium motility, fertility and hatchery rate is 40.84, 68.87 and 47.17%. After storage of 36 days in LN2, the motility of thawn sperm can still attain 3 8.54%. Motility and survival rate of 61.37 and 6 1.4% were obtained in cryopreservation of the spermatozoa of Sparus macrocephalus using a cryoprotectants dilution (DMSO2O%+sucrose5%) and freezing rate 20~C/niin. The volume of samples can affect the result. The nature seawater is better than lower osmotic or high pH activator a solution. The freezing damage occurred at -20扖 to -60扖 for S. macrocephalus. The motility and survival rate of sperm is 50.63% and 61.02% after storaging 66 days in LN2. For sperm of Pagrosomus major the freezing damage occurred at -20扖 to ~60o(2. The nature seawater is a kind suitable activator solution. The optimal motility and survival rate of 50.73 and 62.5% were obtained in cryopreservation of the spermatozoa of P. major using a cryoprotectants dilution (DMSO2O%+sucrose5%) and freezing rate 20C/inin. The motility and survival rate of sperm is 50.63% and 61.02% after storaging 66 days in LN2. In present paper, experimental results of cryopreserving 3 kinds of shellfishs and 2 kinds of fishes are presented and the optimal storing conditions are obtained. And the simplized model of action...