A Study On Transferring Acc Deaminase Gene In Cucumis Melo L.of Xinjiang

A Study on Transferring Acc Deaminase Gene in Cucumis melo L. of Xinjiang Abstract The system of Somatic regenerative plants of Cucumis melo I.. of Xinjiang Was firstly established through researching some factors which could affect plant regeneration such as Hormone levels, cotyledon age, type of explants, light time, culture temperature and so on. As following, an efficient Agrobacterium-mediated transfer system expressing Acc deaminase gene has been developed for melon after the factors concluding an analogue of kanamycin, Ampicilin and time of infection and co-culture influencing transfonnation were carefully investigated. Using the protocol, cotyledonary explants of 8-day-old cucumis melo cv. 揑Fluanghou, Jiashigua, Hongmicui?in virto were infected and transfered by Agrobacterium tumefaciens. The results showed that initial buds have been differentiated from cotyledonary explants cultured onto MS medium supplemented with 1 .0mg/I. BA, 75mg/I. kanamycin and 200mg/I. ampicilin at 27? 揅 under 20001x in 20 days; Subculture of the buds onto MS medium supplemented with 0.1 mg/I. IBA, 50mg/I. kananiycin, 200mg/I. ampiciin without BA caused the development of roots . This is so called ?regeneration plant? The regeneration plants were confirmed as transgenic plants by PCR amplification transgene fragments. The results indicated that the 1kb Ace deaminase gene had integrated within the genome of the regenerative plants.