A Study On Transferring Of Snowdrop Galanthus Nivalis Agglutinin(GNA) Gene In Cucumis Melo L.of Xinjiang

A Study on Transferring Snowdrop Galanthus nivalisagglutinin (GNA) Gene in Cucumis melo L.ofXinjiangAbstracta high efficiency regeneration system of cotyledon explants in Cucumis melo L. Var.Sacchranus.Naus was developed based on the study of the composition of medium, the time and the position of sampling. The results indicated that when the basic cotyledons of 4 day were placed on the bud differentiation medium MS+6-BA1.Omg/L+AgNO3(with 3% sucrose and 0.7% agar ,pH=5.8).The highest differentiation frequencies of 97.5% (jiashi) and 91 %(huanghou)could be obtainded from cotyledon explants, respictively. Furthermore, some factors affecting the transformation frequency, i.e. the kind and concentrations of antibiotics, precultu.re of cotyledon explants, treatments of Agrobacterium and infection time,co-infection time were also discussed. The kanarnycin-resistant plants have been regenerated.They survived after transplantation and grew well in pots.Transgenic plants were confirmed by the kanamycin-resistant experiment, GUS gene and PCR analysis.The preliminary results from bioassay demonstrated significant resistance of the transgenic plants to cotton aphide larvae.