Studies On Inactivated Oil-Emulsion Vaccine Against Avian Viral Arthritis

The characteristics of propagation of four local isolated strains of avian viral arthritis virus(named B.C.G.T strain respectively) in chicken embryo culture. Chicken embryo fibroblast(CEF) and baby hamster kidney cells(BHK-2 1) were compared and studied in this article. The result indicated that the local isolated four strains of AVAV could adapt to chicken embryo culture and chicken embryo fibroblast easily. Between them, the AVAV content on CEF were more than that of chicken embryo culture,and the cytopathic effect on CEF could be observed easily. But after being blind-passaged for 7 generations,the four strains still could not adapt to BHK-2 1. The inactivated experiments were performed using formaldehyde and BET. The results showed that the inactivating effect of 2%o of formaldehyde was better than that of 1% of BET and BEI couldn't inactivate the AVAV thoroughly. By selecting the four strains of AVAV as seed viruses, we developed inactivated Oil-Emulsion vaccine of AVAV. After being tested, the characteristics of physical and infertile test of this kind of were all qualified. 1 Od chickens were vaccined with this kind of vaccine by 1 ml per chicken and no bad effects were observed. This proved the vaccine was safe and reliable. 15 and 30 days after vaccined, the chickens were infected with AVAV and the protective rates were 85% and 90%respectively. The progenies of the vaccined breeding fowls were infected with AVAV at the age of 5 days and 20days , The protective rates were 80% and 53.3% respectively.