Expression And Regulation Of Basigin Gene In Mouse Uterus And Ovary

Basigin, belonging to the immunoglobulin superfamily, has been proved to play an important role in mouse spermatogenesis and early pregnancy. In this study, the expression of basigin gene in mouse uterus of early pregnancy was first systematically analyzed by iminunohistochemistry. The expression and regulation of basigin gene in mouse uterus was also studied under the condition of pseudopregnancy, delayed implantation, artifical decidulization and steroid hormone treatment. In addition, the expressive pattern of basigin gene was also detected in the period of follicle development, the formation and regression of Corpus Luteum(CL) by the methods of immunohistochemistry and in situ hybridization. Basigin immunostaining was very strong in luminal epithelium and glandular epithelium of mouse uterus on day 1 of pregnancy. Then basigin immunostaining was weakening gradually. It was hard to detect the basigin immunostaining on day 4 of pregnancy. The strong staining was seen in the embryos from day 5 to day 8 of pregnancy. On day 5 of pregnancy, basigin was only been seen in embryoes and luminal epithelium and sub-luminal stroma surrounding the blastocyst. A low level of basigin staining was detected in the secondary decidualized cells from day 6 to day 8 of pregnancy. During the estrous cycle, the strongest staining was seen in luminal and glandular epithelium at the estrous stage. Estrogen significantly stimulated the expression of basigin both in luminal and glandular epithelium. The expression of basigin inRNA and protein was becoming stronger as the follicles developed with the stimulation of PMSG and hCG. There were no expression of basigin mRNA and protein in primordial and primary follicles. The first staining of basigin inRNA and protein was detected in the secondary follicles, and the strongest staining was seen in granular cells including cumulus cells of antral follicles. 2 Basigin j拁3L-~~$ The first basigin immunostaining was detected in the granular cell of secondary follicles on day 20 after birth. The strongest staining was in the follicles on day 25 after birth. The immunostaining on day 30 after birth decreased a little, and was the same as that on day 20 after birth. A strong staining of basigin mRNA was seen in the new CL on days 1 and 2 after ovulation, and a weakening staining was detected in CL on days 3 to 5 after ovulation. There was no expression of basigin mRNA after day 7 after ovulation. The expressive mode of basigin mRNA was different from that of basigin protein. Basigin protein was seen in CL on day 1 after ovulation, and the strongest staining was in the CL of day 2 after ovulation. Then the staining was weakening until day 4 after ovulation. There was no staining in CL after day 4 after ovulation. These results showed that the expression of basigin gene might play a very important role in the process of embryo implantation, follicle development and the formation of CL. Postgraduate: Chang Hong Major: Animal Histology and Embryology Supervisor: Yang Zeng-ming...