Study On A Special Gene Marker In A.m.Lindauer Of High Royal Jelly Quantity And Its Appraisal

In this report, 11 random primers (P1, P2, P3, P4, P5, P6, P7, P8, P9, K, W) and RAPD (Random Amplified Polymorphic DNA)-PCR were used to amplify and hereditarily analyze the genomic DNA in four race of Apis mellifera Lindauer (A.m.Lindauer of low royal jelly quantity-carnica; A.m.Lindauer of high royal jelly quantity: Zhenongda , Pinghu and Xiaoshan of A.m.ligustica) which were different in royal jelly quantity. W316bp (bp, base pair) is a common special DNA fragment used PCR with random primer W (5'-CGGCCCCGGT-3') in 3 race of Apis mellifera Lindauer (Zhenongda, Pinghu and Xiaoshan of high royal jelly quantity). W316bp was marked with digoxigenin (DIG). Probe DIG-W316bp hybridized with PCR products and genomic DNA of A.m.Lindauer of high and low royal jelly quantity.The results showed :(1) Primer W produced a special W316bp fragment of DNA which was found in Zhenongda, Pinghu and Xiaoshan of high royal jelly quantity. (2)W316bp labelled with digoxigenin (DIG) may be used to identify special fragment of high or low royal jelly quantity-hybridized by Dot blotting with genomic DNA and Southern blotting with PCR products of A.m.Lindauer. (3) Probe DIG-W316bp only hybridized with 3 races of A.m.Lindauer of high royal jelly quantity and didn't hybridize with A.m.Lindauer of low royal jelly quantity. Bees of high royal jelly quantity appeared the positive signal.The results suggest: It is important that A.m.Lindauer of high royal jelly quantity has the special gene marker-W316bp. It is proved that W316bp is a special DNA fragment in A.m.Lindauer of high royal jelly quantity. W316bp is related to the trait of high royal jelly quantity in A.m.Lindauer. W316bp may be useful to look for the gene, which is related to high royal jelly quantity in A.m.Lindauer.Postgraduate : Zhang Yang-juan Directed by Professor Jiang YingAssociate Professor Wang Wei-ping...