Study On Tissue Culture And Plant Regeneration Of Euphorbia Pulcherrima Willd

This paper reported the research of Euphorbia Pulcherrima Willd used young sterm and young leaves as explants. The MS media was the basic media,given different concentration and ratio auxin or cytokinins. Studied callus induce,endosperm differentiation and reproduce,root differentiation and young plant rootage.Also studied the basic law of reproduction of dormant bud:the effect of different light qualituy and sucrose different concentration.Hope to offer some to the industrialization of Euphorbia Pulcherrima Willd.Different hormone,different concentration could induce different callus.If given same factors,different explants had different callus ratio.If young endosperm was used as explants,the media MS+ZTo.2-i.omg.i_-i(follow as it)+NAAo.s-i.o was the best media.The mediaMS+6-BAi.o-2.o+NAAo.5-2.o seconded to it.If young leaves was used as explants,the mediaMS+6BAi.0-2 o+NAAo.5-2.o was the best media.The media MS+6-BAi.o seconded to it.If the media was given NAA,2,4-D,IAA,callus was loose,moist,milk white or white-green ,transparent or semi- transparent.If the media was given IBA,KT,callus was density,dry,white-green and easy to brown.Callus cultured hi low concentration^l.Omg.L"1) was better than that in high concentration(> 1 .Omg.L"1).The media MS+ZTi.o-2.o+NAAo. 1-0.5 was the best media media for endosperm differentiation.The media MS+6-BAi o-2.o+NAAo.i-o5 seconded to it.Callus cultured in media MS+ZT+NAA was easy to regenerate particulate embryonic callus.On the suffice of callus,there was some cell group,and easy to regenerate plant.Different concentration sugar had different effect to callus.The effect of media with sucrose was better than that with glucan.The concentration of sucrose from 30-40g.L'{ was suitable to callus,the concentration of glucan fromSO-SOg.L"1 was also suitable to callus.At the same time,high concentration sugar inhibited callus induction,but high concentration glucan did not inhibited callus induction.The effect of media MS+IBAoa-i.o+NAAo.i-o.s was best to root differentiation,and media MS+IAAo2-os seconded to it.If media was given IBA,NAA,the root was white,more stronger and grew more quickly,had more root rip,but it would come out callus again.If media was given IAA,root was yellow,slim,less root rip and couldnot come to callus again.The optimum media for amplification was MS+ZTi.o+NAAoj.The mediaMS+ZTi.o+6-BA2.o+NAAo.iseconded to it,but the media MS+6-BA couldnot promote callus induce or differention.Endospenn cultured in media MS+ZTi.0 was strong and its leaves were normal.The optimum media for root regeneration was MS+IAAi.o,and the media MS+IBAo.2+NAAo.5 seconded to it.Contrasted to sun light,callus ratio in red,blue,green and white light was higher,callus ratio in red light was the highest. Light quality had no obvious effect to endosperm differentiation,but green light had a little effect.